دورية أكاديمية
Transforming growth factor-β and toll-like receptor-4 polymorphisms are not associated with fibrosis in haemochromatosis
| العنوان: | Transforming growth factor-β and toll-like receptor-4 polymorphisms are not associated with fibrosis in haemochromatosis |
|---|---|
| المؤلفون: | Wood, Marnie, Powell, Lawrie, Dixon, Jeannette, Subramaniam, V. Nathan, Ramm, Grant |
| المصدر: | World Journal of Gastroenterology |
| بيانات النشر: | WJG Press |
| سنة النشر: | 2013 |
| المجموعة: | Queensland University of Technology: QUT ePrints |
| مصطلحات موضوعية: | 8-oxoguanine DNA glycosylase, Biopsy, Chemokine (C-C motif) ligand 2, Chi-Square Distribution, DNA, DNA glycosyltransferase, Genetic Association Studies, Genetic Predisposition to Disease, Genetic polymorphism, Haemochromatosis, Homozygote, Humans, Interleukin 10, Liver fibrosis, Logistic Models, Monocyte chemoattractant protein 1, Multivariate Analysis, Odds Ratio, Phenotype, Polymorphism, Risk Factors, Severity of Illness Index, Single Nucleotide, Toll-like receptor 4, Transforming growth factor beta, Young Adult, adult, alcohol consumption, article, chemokine receptor CCR2 |
| الوصف: | Aim - To investigate the role of genetic polymorphisms in the progression of hepatic fibrosis in hereditary haemochromatosis. Methods - A cohort of 245 well-characterised C282Y homozygous patients with haemochromatosis was studied, with all subjects having liver biopsy data and DNA available for testing. This study assessed the association of eight single nucleotide polymorphisms (SNPs) in a total of six genes including toll-like receptor 4 (TLR4), transforming growth factor-beta (TGF-β), oxoguanine DNA glycosylase, monocyte chemoattractant protein 1, chemokine C-C motif receptor 2 and interleukin-10 with liver disease severity. Genotyping was performed using high resolution melt analysis and sequencing. The results were analysed in relation to the stage of hepatic fibrosis in multivariate analysis incorporating other cofactors including alcohol consumption and hepatic iron concentration. Results - There were significant associations between the cofactors of male gender (P = 0.0001), increasing age (P = 0.006), alcohol consumption (P = 0.0001), steatosis (P = 0.03), hepatic iron concentration (P < 0.0001) and the presence of hepatic fibrosis. Of the candidate gene polymorphisms studied, none showed a significant association with hepatic fibrosis in univariate or multivariate analysis incorporating cofactors. We also specifically studied patients with hepatic iron loading above threshold levels for cirrhosis and compared the genetic polymorphisms between those with no fibrosis vs cirrhosis however there was no significant effect from any of the candidate genes studied. Importantly, in this large, well characterised cohort of patients there was no association between SNPs for TGF-β or TLR4 and the presence of fibrosis, cirrhosis or increasing fibrosis stage in multivariate analysis. Conclusion - In our large, well characterised group of haemochromatosis subjects we did not demonstrate any relationship between candidate gene polymorphisms and hepatic fibrosis or cirrhosis. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | https://eprints.qut.edu.au/101157/1/WJG-19-9366.pdfTest; Wood, Marnie, Powell, Lawrie, Dixon, Jeannette, Subramaniam, V. Nathan, & Ramm, Grant (2013) Transforming growth factor-β and toll-like receptor-4 polymorphisms are not associated with fibrosis in haemochromatosis. World Journal of Gastroenterology, 19(48), pp. 9366-9376.; https://eprints.qut.edu.au/101157Test/ |
| الإتاحة: | https://doi.org/10.3748/wjg.v19.i48.9366Test https://eprints.qut.edu.au/101157Test/ |
| حقوق: | free_to_read ; http://creativecommons.org/licenses/by-nc/2.5Test/ ; Consult author(s) regarding copyright matters ; This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au |
| رقم الانضمام: | edsbas.84A37DD0 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |