دورية أكاديمية
Hypervirulent and hypermucoviscous extended-spectrum β-lactamase-producing Klebsiella pneumoniae and Klebsiella variicola in Chile
| العنوان: | Hypervirulent and hypermucoviscous extended-spectrum β-lactamase-producing Klebsiella pneumoniae and Klebsiella variicola in Chile |
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| المؤلفون: | F. Morales-León, A. Opazo-Capurro, C. Caro, N. Lincopan, A. Cardenas-Arias, F. Esposito, V. Illesca, M. L. Rioseco, M. Domínguez-Yévenes, C. A. Lima, H. Bello-Toledo, Gerardo González-Rocha |
| المصدر: | Virulence, Vol 12, Iss 1, Pp 35-44 (2021) |
| بيانات النشر: | Taylor & Francis Group |
| سنة النشر: | 2021 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | klebsiella pneumoniae complex, virulence, hypermucoviscous, esbl, multidrug-resistance, Infectious and parasitic diseases, RC109-216 |
| الوصف: | Convergence of virulence and antibiotic-resistance has been reported in Klebsiella pneumoniae, but not in Klebsiella variicola. We, hereby, report the detection and genomic characterization of hypervirulent and hypermucoviscous K. pneumoniae and K.variicola recovered in Chile from health-care associated infections, which displayed resistance to broad-spectrum cephalosporins. One hundred forty-six K. pneumoniae complex isolates were screened by hypermucoviscosity by the “string test.” Two hypermucoid isolates, one hypermucoviscous K. pneumoniae (hmKp) and one K. variicola (hmKv), were further investigated by whole-genome sequencing. In vivo virulence was analyzed by the Galleria mellonella killing assay. In silico analysis of hmKp UCO-494 and hmKv UCO-495 revealed the presence of multiple antibiotic-resistance genes, such as blaCTX-M-1, blaDHA-1 and blaLEN-25 among others clinically relevant resistance determinants, including mutations in a two-component regulatory system related to colistin resistance. These genetic features confer a multidrug-resistant (MDR) phenotype in both strains. Moreover, virulome in silico analysis confirmed the presence of the aerobactin gene iutA, in addition to yersiniabactin and/or colicin V encoding genes, which are normally associated to high virulence in humans. Furthermore, both isolates were able to kill G. mellonella and displayed higher virulence in comparison with the control strain. In summary, the convergence of virulence and the MDR-phenotype in K. pneumoniae complex members is reported for the first time in Chile, denoting a clinical problem that deserves special attention and continuous surveillance in South America. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2150-5594 2150-5608 |
| العلاقة: | http://dx.doi.org/10.1080/21505594.2020.1859274Test; https://doaj.org/toc/2150-5594Test; https://doaj.org/toc/2150-5608Test; https://doaj.org/article/a88728e349d741018b9a1de072be4e93Test |
| DOI: | 10.1080/21505594.2020.1859274 |
| الإتاحة: | https://doi.org/10.1080/21505594.2020.1859274Test https://doaj.org/article/a88728e349d741018b9a1de072be4e93Test |
| رقم الانضمام: | edsbas.4DEF5A78 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 21505594 21505608 |
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| DOI: | 10.1080/21505594.2020.1859274 |