دورية
Human Immunodeficiency Virus Type 1 Nef Epitopes Recognized in HLA-A2 Transgenic Mice in Response to DNA and Peptide Immunization
| العنوان: | Human Immunodeficiency Virus Type 1 Nef Epitopes Recognized in HLA-A2 Transgenic Mice in Response to DNA and Peptide Immunization |
|---|---|
| المؤلفون: | Sandberg, Johan K., Leandersson, Ann-Charlotte, Devito, Claudia, Kohleisen, Birgit, Erfle, Volker, Achour, Adnane, Levi, Michael, Schwartz, Stefan, Kärre, Klas, Wahren, Britta, Hinkula, Jorma |
| المصدر: | Virology; July 2000, Vol. 273 Issue: 1 p112-119, 8p |
| مستخلص: | We investigated the immune response against a human immunodeficiency virus type 1 (HIV-1) nefDNA sequence administered epidermally in mice transgenic for the human major histocompatibility complex (MHC) class I molecule HLA-A201. Ten potential HLA-A2 binding 9-mer Nef peptides were identified by a computer-based search algorithm. By a cell surface MHC class I stabilization assay, four peptides were scored as good binders, whereas two peptides bound weakly to HLA-A2. After DNA immunization, cytotoxic T lymphocyte (CTL) responses were predominantly directed against the Nef 44-52, 81-89, and 85-93 peptides. Interestingly, the 44-52 epitope resides outside the regions of Nef where previously described CTL epitopes are clustered. Dominance among Nef-derived peptides did not strictly correlate with HLA-A2 binding, in that only one of the high-affinity binding peptides was targeted in the CTL response. The 44-52, 85-93, and 139-147 peptides also generated specific CTLs in response to peptide immunization. T helper cell proliferation was detected after stimulation with 20-mer peptides in vitro. Three Nef regions (16-35, 106-125, and 166-185) dominated the T helper cell proliferation. The implications of these results for the development of DNA-based vaccines against HIV is discussed. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 00426822 10960341 |
|---|---|
| DOI: | 10.1006/viro.2000.0360 |