Sinusoidal and pericellular fibrosis in adult post-transplant liver biopsies: association with hepatic stellate cell activation and patient outcome
| العنوان: | Sinusoidal and pericellular fibrosis in adult post-transplant liver biopsies: association with hepatic stellate cell activation and patient outcome |
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| المؤلفون: | Dina Tiniakos, Rachael Saunders, Sameh Abou-Beih, Steven Masson, Fiona Oakley, Beate Haugk |
| المصدر: | Virchows Archiv |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Adult, Liver Cirrhosis, Male, 0301 basic medicine, Pathology, medicine.medical_specialty, Biopsy, medicine.medical_treatment, Liver fibrosis, Inflammation, Liver transplantation, medicine.disease_cause, Pathology and Forensic Medicine, 03 medical and health sciences, Ballooning degeneration, 0302 clinical medicine, Fibrosis, Hepatic Stellate Cells, medicine, Humans, Molecular Biology, Centrilobular fibrosis, Aged, Retrospective Studies, Alpha-smooth muscle actin, business.industry, Sonic hedgehog, Cell Biology, General Medicine, Middle Aged, medicine.disease, Hepatic stellate cell activation, 3. Good health, Treatment Outcome, 030104 developmental biology, 030220 oncology & carcinogenesis, Hepatic stellate cell, Original Article, Female, medicine.symptom, business, Immunostaining, Oxidative stress |
| الوصف: | Post-transplant sinusoidal fibrosis (SF) and pericellular fibrosis (PCF) have not been extensively investigated in adults. Fifty-two post-transplant liver biopsies from 28 consented patients (12 men, mean age 49, range 33–67 years) were studied. Tissue morphology, including an arbitrary summative fibrosis score was assessed in detail. Collagen proportionate area (CPA) and alpha-smooth muscle actin (α-SMA) immunostain were evaluated by digital image analysis (DIA). Anti-keratin 7, anti-C4d and anti-sonic hedgehog (Shh) immunostains were scored semi-quantitatively. SF was observed in 36/52 (69.2%) biopsies and most of these (20/36, 55.6%) had centrilobular fibrosis (CLF). PCF was seen in 7/52 (13.5%) biopsies exclusively in cases with CLF. CPA was significantly correlated with time since liver transplantation (p = 0.043), summative fibrosis score and its main components but not with α-SMA. α-SMA-positive area significantly correlated with the Banff rejection score (p = 0.022) and centrilobular inflammatory changes were more severe in cases with CLF (p = 0.003). Hepatocyte ballooning of cholestatic type was associated with PCF (p = 0.016) and Shh expression (p |
| تدمد: | 1432-2307 0945-6317 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::02752dfbf0cad47c78676e1986454ee6Test https://doi.org/10.1007/s00428-019-02585-xTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....02752dfbf0cad47c78676e1986454ee6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14322307 09456317 |
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