التفاصيل البيبلوغرافية
| العنوان: |
Characterization of two novel porcine reproductive and respiratory syndrome virus isolates with deletions in the GP2 gene. |
| المؤلفون: |
Chen, Jia-zeng1, Peng, Jin-mei1, Bai, Yun1, Wang, Qian1, Liu, Yi-min1, Zhang, Qiu-yue1, Chang, Dan1, Zhang, Wu-chao1, Zhao, Hong-yuan1, Ye, Chao1, An, Tong-qing1, Cai, Xue-hui1, Tian, Zhi-jun1 tzj@hvri.ac.cn, Tong, Guang-zhi2 gztong@shvri.ac.cn |
| المصدر: |
Veterinary Microbiology. Apr2015, Vol. 176 Issue 3/4, p344-351. 8p. |
| مصطلحات موضوعية: |
*PORCINE reproductive & respiratory syndrome, *DELETION mutation, *SOWS, *ALVEOLAR macrophages, *NUCLEOTIDE sequence, *ANTISENSE DNA, *DISEASES, *VIRUSES |
| مستخلص: |
Two newly emerged, porcine reproductive and respiratory syndrome virus (PRRSV) strains (Henan-A10 and A11) were isolated from the sera of aborting sows. Interestingly, both of the isolates could replicate in primary porcine alveolar macrophage (PAM) cells but not in MARC-145 cells. A phylogenetic tree based on the complete genome was constructed and the results showed that Henan-A10 and A11 were most closely related to other highly pathogenic PRRSV (HP-PRRSV) strains. However, genomic sequence analysis showed that Henan-A10 and A11 shared only 96.8–97.8% nucleotide identity with the representative HP-PRRSV strain JXA1. Notably, a 10 amino acids deletion in the GP2 endodomain was identified for the first time. A full-length, infectious cDNA clone of HuN4-F112 (attenuated strain from a HP-PRRSV) was used to construct a chimeric clone with the corresponding deletion in GP2. We found that the deletion did not affect viral growth in MARC-145 cells, indicating that the endodomain of PRRSV GP2 may be variable. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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