المؤلفون: Li Pan, Chen Ji, Mengnan Qi, Bing Wang, Tao Wang, Yuzi Luo, Hua-Ji Qiu, Rui Luo, Yuan Sun, Maowen Sun

المصدر: Veterinary Microbiology. 263:109245

مصطلحات موضوعية: Swine Diseases, General Veterinary, biology, African swine fever, Swine, Disinfectant, chemistry.chemical_element, General Medicine, biology.organism_classification, Iodine, African Swine Fever Virus, Microbiology, African swine fever virus, Virology, chemistry, Animals, Virus Inactivation, Animal Husbandry, African Swine Fever, Povidone-Iodine, Disinfectants

الوصف: African swine fever (ASF) is a highly lethal contagious disease of swine caused by African swine fever virus (ASFV). Cleaning and disinfection remain one of the most effective tools to prevent the ASFV spread in pig holdings. This study evaluated the inactivation effect of a highly complexed iodine (HPCI) disinfectant against ASFV. A commercially available povidone-iodine (PVP-I) was used as reference for comparison. The results showed that 5% HPCI and 5% PVP-I did not exhibit cytotoxicity in primary porcine alveolar macrophages, and 107.0 and 105.0 TCID50/mL ASFV were completely inactivated by 5% and 0.25% HPCI, respectively, in 5 min via either immersion or spray disinfection. However, 5% PVP-I required at least 15 min to completely inactivate 107.0 TCID50/mL ASFV, whereas 0.25% PVP-I failed to completely inactivate 105.0 TCID50/mL ASFV. This study demonstrated that HPCI could rapidly and efficiently inactivate ASFV, representing an effective disinfectant for ASF control.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a343ead421ae098ec26c25856a27a67eTest
https://doi.org/10.1016/j.vetmic.2021.109245Test

المؤلفون: Li Zhang, Xiangdong Jia, Yuzi Luo, Hua-Ji Qiu, Wei Wang, Yuan Sun, Madiha Fatima, Jiaoer Zhang

المصدر: Veterinary Microbiology

مصطلحات موضوعية: 2019-20 coronavirus outbreak, Swine, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Disinfectant, Microbiology, African swine fever virus, Article, Inactivation, Virus, 03 medical and health sciences, Ozone, Macrophages, Alveolar, Animals, African Swine Fever, Cells, Cultured, 030304 developmental biology, Infectivity, Swine Diseases, 0303 health sciences, General Veterinary, biology, African swine fever, 030306 microbiology, Water, General Medicine, biology.organism_classification, Ozonized water, African Swine Fever Virus, Titer, Virus Inactivation, Disinfectants

الوصف: Highlights • Inactivation effects of ozonized water on ASFV was firstly evaluated. • ASFV can be rapidly and efficiently inactivated by ozonized water. • Ozonized water can be used as an effective disinfectant for ASF control.
African swine fever virus (ASFV) is the causative agent of African swine fever (ASF), which is a devastating disease of domestic pigs and wild boar, causing significant economic losses to the pig industry worldwide. To evaluate the ability of ozonized water as a disinfectant to inactivate ASFV, ozonized water of different concentrations was tested, and the viral reduction was determined by infectivity assay on porcine primary alveolar macrophages. The results showed that 2 log10 (99 %) reduction in viral titer was observed when 104.0 TCID50/mL wild-type or reporter ASFV was inactivated with ozonized water as lower as 5 mg/L within 1 min at room temperature; while a viral reduction of approximately 2 log10 (99 %) was observed when 105.0 TCID50/mL wild-type or reporter ASFV was inactivated with 5 mg/L ozonized water within 1 min, and 3 log10 (99.9 %) virus was inactivated by 10 or 20 mg/L ozonized water within 3 or 1 min, respectively; furthermore, 5 mg/L ozonized water inactivated 2 log10 (99 %) reporter ASFV as higher as 106.75 TCID50/mL in 1 min, and a viral reduction of approximately 3 log10 (99.9 %) in reporter ASFV or 2 log10 (99 %) in wild-type virus was observed when inactivated with 10 mg/L ozonized water in 1 min; meanwhile, a viral reduction of 3 log10 (99.9 %) was observed when 20 mg/L ozonized water was applied to the wild-type ASFV of 106.75 TCID50/mL in 3 min. Overall, ozonized water can rapidly and efficiently inactivate ASFV, representing an effective disinfectant for ASF control.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0227c8438bc4a08555a5ad986fbdf939Test
https://pubmed.ncbi.nlm.nih.gov/32768237Test

المؤلفون: Pan, Li¹ (AUTHOR), Luo, Rui^1,2 (AUTHOR), Wang, Tao¹ (AUTHOR), Qi, Mengnan¹ (AUTHOR), Wang, Bing¹ (AUTHOR), Sun, Maowen¹ (AUTHOR), Luo, Yuzi¹ (AUTHOR), Ji, Chen³ (AUTHOR), Sun, Yuan¹ (AUTHOR) sunyuan@caas.cn, Qiu, Hua-Ji^1,2 (AUTHOR) qiuhuaji@caas.cn

المصدر: Veterinary Microbiology. Dec2021, Vol. 263, pN.PAG-N.PAG. 1p.

مصطلحات موضوعية: *AFRICAN swine fever virus, *AFRICAN swine fever, *IODINE, *SWINE diseases, *COMMUNICABLE diseases, *ALVEOLAR macrophages

مستخلص: • Highly complexed iodine exhibited lower cytotoxicity than traditional povidone-iodine. • Highly complexed iodine completely inactivated ASFV within 5 min. • Highly complexed iodine effectively inactivated ASFV via immersion or spray disinfection. African swine fever (ASF) is a highly lethal contagious disease of swine caused by African swine fever virus (ASFV). Cleaning and disinfection remain one of the most effective tools to prevent the ASFV spread in pig holdings. This study evaluated the inactivation effect of a highly complexed iodine (HPCI) disinfectant against ASFV. A commercially available povidone-iodine (PVP-I) was used as reference for comparison. The results showed that 5% HPCI and 5% PVP-I did not exhibit cytotoxicity in primary porcine alveolar macrophages, and 107.0 and 105.0 TCID 50 /mL ASFV were completely inactivated by 5% and 0.25% HPCI, respectively, in 5 min via either immersion or spray disinfection. However, 5% PVP-I required at least 15 min to completely inactivate 107.0 TCID 50 /mL ASFV, whereas 0.25% PVP-I failed to completely inactivate 105.0 TCID 50 /mL ASFV. This study demonstrated that HPCI could rapidly and efficiently inactivate ASFV, representing an effective disinfectant for ASF control. [ABSTRACT FROM AUTHOR]