Efficient inactivation of African swine fever virus by ozonized water
| العنوان: | Efficient inactivation of African swine fever virus by ozonized water |
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| المؤلفون: | Li Zhang, Xiangdong Jia, Yuzi Luo, Hua-Ji Qiu, Wei Wang, Yuan Sun, Madiha Fatima, Jiaoer Zhang |
| المصدر: | Veterinary Microbiology |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 2019-20 coronavirus outbreak, Swine, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Disinfectant, Microbiology, African swine fever virus, Article, Inactivation, Virus, 03 medical and health sciences, Ozone, Macrophages, Alveolar, Animals, African Swine Fever, Cells, Cultured, 030304 developmental biology, Infectivity, Swine Diseases, 0303 health sciences, General Veterinary, biology, African swine fever, 030306 microbiology, Water, General Medicine, biology.organism_classification, Ozonized water, African Swine Fever Virus, Titer, Virus Inactivation, Disinfectants |
| الوصف: | Highlights • Inactivation effects of ozonized water on ASFV was firstly evaluated. • ASFV can be rapidly and efficiently inactivated by ozonized water. • Ozonized water can be used as an effective disinfectant for ASF control. African swine fever virus (ASFV) is the causative agent of African swine fever (ASF), which is a devastating disease of domestic pigs and wild boar, causing significant economic losses to the pig industry worldwide. To evaluate the ability of ozonized water as a disinfectant to inactivate ASFV, ozonized water of different concentrations was tested, and the viral reduction was determined by infectivity assay on porcine primary alveolar macrophages. The results showed that 2 log10 (99 %) reduction in viral titer was observed when 104.0 TCID50/mL wild-type or reporter ASFV was inactivated with ozonized water as lower as 5 mg/L within 1 min at room temperature; while a viral reduction of approximately 2 log10 (99 %) was observed when 105.0 TCID50/mL wild-type or reporter ASFV was inactivated with 5 mg/L ozonized water within 1 min, and 3 log10 (99.9 %) virus was inactivated by 10 or 20 mg/L ozonized water within 3 or 1 min, respectively; furthermore, 5 mg/L ozonized water inactivated 2 log10 (99 %) reporter ASFV as higher as 106.75 TCID50/mL in 1 min, and a viral reduction of approximately 3 log10 (99.9 %) in reporter ASFV or 2 log10 (99 %) in wild-type virus was observed when inactivated with 10 mg/L ozonized water in 1 min; meanwhile, a viral reduction of 3 log10 (99.9 %) was observed when 20 mg/L ozonized water was applied to the wild-type ASFV of 106.75 TCID50/mL in 3 min. Overall, ozonized water can rapidly and efficiently inactivate ASFV, representing an effective disinfectant for ASF control. |
| تدمد: | 1873-2542 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0227c8438bc4a08555a5ad986fbdf939Test https://pubmed.ncbi.nlm.nih.gov/32768237Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0227c8438bc4a08555a5ad986fbdf939 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18732542 |
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