التفاصيل البيبلوغرافية
| العنوان: |
Pax5 immunostaining in paraffin-embedded sections of canine non-Hodgkin lymphoma: A novel canine pan pre-B- and B-cell marker |
| المؤلفون: |
Willmann, M.1 michael.willmann@vu-wien.ac.at, Müllauer, L.2, Guija de Arespacochaga, A.3, Reifinger, M.4, Mosberger, I.2, Thalhammer, J.G.1 |
| المصدر: |
Veterinary Immunology & Immunopathology. Apr2009, Vol. 128 Issue 4, p359-365. 7p. |
| مصطلحات موضوعية: |
*GENES, *STAINS & staining (Microscopy), *PARAFFIN wax, *LYMPHOMAS in animals, *DOG diseases, *B cells, *TUMOR markers, *IMMUNOHISTOCHEMISTRY |
| مستخلص: |
Abstract: The Pax5 gene encodes the B-cell specific activator protein (BSAP), a member of the highly conserved paired box (PAX)-domain family of transcription factors and a key regulator in the development and differentiation of B-cells. Pax5 serves as a valuable B-cell marker in the classification of human lymphoma patients as it is restricted to lymphomas of B-cell lineage. In dogs, detection of Pax5 protein in lymphoma tissue has not been reported. Therefore, we have investigated the expression and detection of BSAP using a monoclonal anti-Pax5 antibody (anti-BSAP, clone 24) in canine lymphoma tissue samples to evaluate its diagnostic relevance as a B-cell marker. A series of 25 lymph nodes from 23 canine non-Hodgkin lymphoma patients, a reactive canine lymph node, and a normal non-reactive canine lymph node, were evaluated. All B-cell non-Hodgkin lymphomas (15) were found to express Pax5 protein. In addition, there was a strong correlation between Pax5 and CD79a expression. Three CD3 positive and five CD3 and CD79a positive lymphomas were immunophenotypically negative for anti-Pax5, indicating a T-cell lineage. In conclusion, anti-Pax5 antibody may offer an excellent B-cell marker in canine lymphomas. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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