دورية أكاديمية
Development and validation of an antigen capture ELISA based on monoclonal antibodies specific for Listeria monocytogenes in food
| العنوان: | Development and validation of an antigen capture ELISA based on monoclonal antibodies specific for Listeria monocytogenes in food |
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| المؤلفون: | Rossella Lelli, Cinzia Pompilii, Mirella Luciani, Tiziana Di Febo, Ottavio Portanti, Primula Semprini |
| المصدر: | Veterinaria Italiana, Vol 47, Iss 3, Pp 281-290 (2011) |
| بيانات النشر: | Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale, 2011. |
| سنة النشر: | 2011 |
| المجموعة: | LCC:Animal culture LCC:Veterinary medicine |
| مصطلحات موضوعية: | Capture ELISA, Enzyme-linked immunosorbent assay, ELISA, Food, Hygiene, International Organization for Standardization, Italy, Listeria monocytogenes, Animal culture, SF1-1100, Veterinary medicine, SF600-1100 |
| الوصف: | A capture enzyme-linked immunosorbent assay (ELISA) for the identification of Listeria monocytogenes in food was standardised and validated. The assay was refined by analysing samples of meat, seafood, dairy products, pasta and flour. The method was found to be 100% specific for Listeria spp. tested, with a limit of sensitivity of 6.6 × 10(3) colony-forming units (cfu)/ml. Comparison of L. monocytogenes capture ELISA against the official International Organization for Standardization (ISO) method 11290-1:1996 for the isolation and identification of L. monocytogenes in food matrices produced a significant concordance index. The assay was validated on food matrices including meat, seafood and dairy products in line with ISO 16140:2003 concerning qualitative analytical methods. The assay was found to be accurate, specific, sensitive, selective, reproducible and fast, resulting in lower costs and faster turnaround in microbiological screening of foods. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0505-401X 1828-1427 |
| العلاقة: | http://www.izs.it/vet_italiana/2011/47_3/281.pdfTest; https://doaj.org/toc/0505-401XTest; https://doaj.org/toc/1828-1427Test |
| الوصول الحر: | https://doaj.org/article/88b515fda5ee41d1ba7f3aa2bea5772bTest |
| رقم الانضمام: | edsdoj.88b515fda5ee41d1ba7f3aa2bea5772b |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 0505401X 18281427 |
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