دورية أكاديمية
Human transcriptomic response to the VSV-vectored Ebola vaccine
| العنوان: | Human transcriptomic response to the VSV-vectored Ebola vaccine |
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| المؤلفون: | Santoro, F., Donato, A., Lucchesi, S., Sorgi, S., Gerlini, A., Haks, M.C., Ottenhoff, T.H.M., Gonzalez-Dias, P., Nakaya, H.I., Huttner, A., Siegrist, C.A., Medaglini, D., Pozzi, G., VSV-EBOVAC Consortium, VSV-EBOPLUS Consortium |
| المصدر: | Vaccines |
| سنة النشر: | 2021 |
| المجموعة: | Leiden Repository (Leiden University) |
| مصطلحات موضوعية: | Ebolavirus Disease, transcriptomics, recombinant VSV, VSV-ZEBOV, vaccine, live viral vector |
| الوصف: | Ebolavirus Disease (EVD) is a severe haemorrhagic fever that occurs in epidemic outbreaks, with a high fatality rate and no specific therapies available. rVSV Delta G-ZEBOV-GP (Ervebo(R)), a live-attenuated recombinant vesicular stomatitis virus vector expressing the glycoprotein G of Zaire Ebolavirus, is the first vaccine approved for prevention of EVD. Both innate and adaptive responses are deemed to be involved in vaccine-induced protection, yet the mechanisms are not fully elucidated. A global transcriptomic approach was used to profile the blood host-response in 51 healthy volunteers enrolled in a phase 1/2 clinical trial. Signatures of the host responses were investigated assessing the enrichment in differentially expressed genes (DEGs) of specific "blood transcription modules" (BTM). Comparison of gene-expression levels showed that vaccination produces a peak of 5469 DEGs at day one, representing 38.6% of the expressed genes. Out of 346 BTMs, 144 were significantly affected by vaccination. Innate immunity pathways were induced from day 1 to day 14. At days 2 and 3, neutrophil modules were downregulated and complement-related modules upregulated. T-cell and cell-cycle associated modules were upregulated at days 7 and 14, while at day 28, no modules remained activated. At day 14, a direct correlation was observed between ZEBOV glycoprotein-specific antibody titres and activation of seven BTMs, including two related to B-cell activation and B cell receptor signalling. Transcriptomic analysis identified an rVSV Delta G-ZEBOV-GP-induced signature and demonstrated a direct correlation of blood transcriptomic changes with ZEBOV glycoprotein-specific antibody titres. ; Immunogenetics and cellular immunology of bacterial infectious diseases |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://www.mdpi.com/2076-393X/9/2/67/pdfTest; lumc-id: 116353100; https://hdl.handle.net/1887/3196253Test |
| DOI: | 10.3390/vaccines9020067 |
| الإتاحة: | https://doi.org/10.3390/vaccines9020067Test https://hdl.handle.net/1887/3196253Test https://www.mdpi.com/2076-393X/9/2/67/pdfTest |
| رقم الانضمام: | edsbas.DF2200F0 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/vaccines9020067 |
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