Alphaviral vector-transduced dendritic cells are successful therapeutic vaccines against neu-overexpressing tumors in wild-type mice
| العنوان: | Alphaviral vector-transduced dendritic cells are successful therapeutic vaccines against neu-overexpressing tumors in wild-type mice |
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| المؤلفون: | Jonathan S. Serody, Ivana R. Ferrer, Timothy P. Moran, Robert E. Johnston, Roland Tisch, Elizabeth M. Jaffee, Joseph E. Burgents, Brian Long |
| المصدر: | Vaccine. 25(36) |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | CD4-Positive T-Lymphocytes, Receptor, ErbB-2, T-Lymphocytes, medicine.medical_treatment, T cell, Genetic Vectors, Alphavirus, CD8-Positive T-Lymphocytes, Biology, Cancer Vaccines, Article, Cell Line, Viral vector, Proinflammatory cytokine, Mice, Lymphocytes, Tumor-Infiltrating, medicine, Animals, Humans, B cell, General Veterinary, General Immunology and Microbiology, Public Health, Environmental and Occupational Health, 3T3 Cells, Dendritic Cells, Neoplasms, Experimental, Immunotherapy, Dendritic cell, Flow Cytometry, Infectious Diseases, medicine.anatomical_structure, Cell culture, Immunoglobulin G, Immunology, NIH 3T3 Cells, Cancer research, Molecular Medicine, Female, CD8 |
| الوصف: | While dendritic cell (DC) vaccines can protect hosts from tumor challenge, their ability to effectively inhibit the growth of established tumors remains indeterminate. Previously, we have shown that human DCs transduced with Venezuelan equine encephalitis virus replicon particles (VRPs) were potent stimulators of antigen-specific T cells in vitro. Therefore, we investigated the ability of VRP-transduced DCs (VRP-DCs) to induce therapeutic immunity in vivo against tumors overexpressing the neu oncoprotein. Transduction of murine DCs with VRPs resulted in high-level transgene expression, DC maturation and secretion of proinflammatory cytokines. Vaccination with VRP-transduced DCs (VRP-DCs) expressing a truncated neu oncoprotein induced robust neu-specific CD8+ T cell and anti-neu IgG responses. Furthermore, a single vaccination with VRP-DCs induced the regression of large established tumors in wild-type mice. Interestingly, depletion of CD4+, but not CD8+, T cells completely abrogated inhibition of tumor growth following vaccination. Taken together, our results demonstrate that VRP-DC vaccines induce potent immunity against established tumors, and emphasize the importance of the generation of both CD4+ T cell and B cell responses for efficient tumor inhibition. These findings provide the rationale for future evaluation VRP-DC vaccines in the clinical setting. |
| اللغة: | English |
| تدمد: | 1873-2518 0264-410X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4fbe91a475a83078fe3ca412ccedddfeTest http://ora.ox.ac.uk/objects/uuid:e422ac33-3bec-4a0e-9a2d-f675e625cd0bTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4fbe91a475a83078fe3ca412ccedddfe |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18732518 0264410X |
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