Protection of red-legged partridges (Alectoris rufa) against West Nile virus (WNV) infection after immunization with WNV recombinant envelope protein E (rE)
| العنوان: | Protection of red-legged partridges (Alectoris rufa) against West Nile virus (WNV) infection after immunization with WNV recombinant envelope protein E (rE) |
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| المؤلفون: | Miguel A. Martín-Acebes, Teresa Merino-Ramos, Estela Escribano-Romero, N. Jiménez de Oya, Juan-Carlos Saiz, Ursula Höfle, Virginia Gamino, José M. Escribano, Ana Valeria Gutiérrez-Guzmán, Ana-Belén Blázquez |
| المساهمون: | CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Network of Animal Disease Infectiology Research Facilities, European Commission, Consejo Superior de Investigaciones Científicas (España) |
| المصدر: | Repositorio de Resultados de Investigación del INIA INIA: Repositorio de Resultados de Investigación del INIA Digital.CSIC. Repositorio Institucional del CSIC instname Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA |
| بيانات النشر: | Elsevier, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | 040301 veterinary sciences, Partridges, visual_art.art_subject, animal diseases, Oropharynx, Antibodies, Viral, Injections, Intramuscular, 0403 veterinary science, Birds, 03 medical and health sciences, Cloaca, Viral Envelope Proteins, Animals, Transmission, West Nile Virus Vaccines, Galliformes, 030304 developmental biology, Shedding, Bird Diseases, 0303 health sciences, Vaccines, Synthetic, Protection, General Veterinary, General Immunology and Microbiology, biology, Vaccination, Public Health, Environmental and Occupational Health, 04 agricultural and veterinary sciences, biology.organism_classification, Virology, Survival Analysis, Alectoris rufa, Recombinant Proteins, 3. Good health, Infectious Diseases, Blood, Feather, visual_art, visual_art.visual_art_medium, Molecular Medicine, Enzootic, West Nile virus, Vaccine, West Nile Fever |
| الوصف: | West Nile virus (WNV) is maintained in nature in an enzootic transmission cycle between birds and mosquitoes, although it occasionally infects other vertebrates, including humans, in which it may result fatal. To date, no licensed vaccines against WNV infection are available for birds, but its availability would certainly benefit certain populations, as birds grown for restocking, hunting activities, or alimentary purposes, and those confined to wildlife reservations and recreation installations. We have tested the protective capability of WNV envelope recombinant (rE) protein in red-legged partridges (Alectoris rufa). Birds (n=28) were intramuscularly immunized three times at 2-weeks interval with rE and a control group (n=29) was sham-immunized. Except for 5 sham-immunized birds that were not infected and housed as contact controls, partridges were subcutaneously challenged with WNV. Oropharyngeal and cloacal swabs and feather pulps were collected at several days after infection and blood samples were taken during vaccination and after infection. All rE-vaccinated partridges elicited anti-WNV antibodies before challenge and survived to the infection, while 33.3% of the sham-immunized birds succumbed, as did 25% of the contact animals. Most (84%) unvaccinated birds showed viremia 3 d.p.i., but virus was only detected in 14% of the rE vaccinated birds. WNV-RNA was detected in feathers and swabs from sham-immunized partridges from 3 to 7 d.p.i., mainly in birds that succumbed to the infection, but not in rE vaccinated birds. Thus, rE vaccination fully protected partridges against WND and reduced the risk of virus spread. This work was supported by grants FAU2008-00006 and RTA2011-00036 from the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) and by the Network of Animal Disease Infectiology and Research-European Union (NADIR-EU-228394). M.A.M.A. is a recipient of a “Junta de Ampliación de Estudios (JAE)” doctoral fellowship from the Spanish Research Council (CSIC). T.M.R. is a recipient of a “Formación de Personal Investigador (FPI)” pre-doctoral fellowship from INIA. |
| تدمد: | 1873-2518 0264-410X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f26b690964a84a501048aa784e7168dTest http://hdl.handle.net/10261/142914Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3f26b690964a84a501048aa784e7168d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18732518 0264410X |
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