التفاصيل البيبلوغرافية
| العنوان: |
Nonviral therapeutic cell vaccine mediates potent antitumor effects |
| المؤلفون: |
Moret-Tatay, Inés1, Sanmartín, Isaías1, Marco, Francisco M.2, Díaz, Joaquín2, Aliño, Salvador F.1 alino@uv.es |
| المصدر: |
Vaccine. May2006, Vol. 24 Issue 18, p3937-3945. 9p. |
| مصطلحات موضوعية: |
*VACCINATION, *PREVENTIVE medicine, *TUMORS, *CELLS |
| مستخلص: |
Abstract: Therapeutic vaccination of mice bearing melanoma tumors with our genetically modified tumor cells, via DOTAP/GM-CSF lipoplexes, results in >85% tumor growth inhibition. These fresh transfected cells (irradiated, frozen and thawed) are able to produce high amounts of GM-CSF transgene (>200ng/106 cells/24h). After vaccination, significant increases (>eight-fold) in specific antitumor membrane protein IgG1 and IgG2a are obtained only in groups vaccinated with GM-CSF-producing cells, where also the highest rates of tumor inhibition, and significantly delayed mice death (P <0.05), are observed. The antitumor response obtained is long-lasting in survivors (GM-CSF-group) from 6 months after the first tumor challenge, and a full 100% of mice survived to a second tumor challenge. All these results suggest that antitumor cell vaccines engineered by nonviral procedures are suitable for use as therapeutic vaccines with potential clinical applications. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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