التفاصيل البيبلوغرافية
| العنوان: |
Development of a subunit vaccine containing recombinant Riemerella anatipestifer outer membrane protein A and CpG ODN adjuvant. |
| المؤلفون: |
Chu, Chun-Yen1, Liu, Chia-Hui1, Liou, Jhong-Jie1, Lee, Jai-Wei2, Cheng, Li-Ting1 chenglt@mail.npust.edu.tw |
| المصدر: |
Vaccine. Jan2015, Vol. 33 Issue 1, p92-99. 8p. |
| مصطلحات موضوعية: |
*GRAM-negative bacterial diseases, *BACTERIAL cell walls, *MEMBRANE proteins, *BACTERIAL vaccines, *CPG nucleotides, *SEPSIS, *DUCKS, *DISEASES |
| مستخلص: |
Riemerella anatipestifer , a Gram-negative bacillus, causes septicemia that can result in high mortality for ducklings. In this study, we evaluated the immune response and protective efficacy provided by a subunit vaccine containing recombinant outer membrane protein A (rOmpA) and plasmid constructs containing CpG oligodeoxynucleotides (ODN). Results showed that CpG ODN enhanced both humoral and cell-mediated immunity elicited by rOmpA as early as two weeks after primary immunization. When compared to ducks immunized with rOmpA, ducks immunized with rOmpA + CpG ODN showed higher levels ( p < 0.05) of antibody titer, T cell proliferation, and percentages of CD4 + and CD8 + T cell in peripheral blood mononuclear cells (PBMCs). The relative fold inductions of mRNA expression of Th1-type (IFN-γ and IL-12), and Th2-type (IL-6) cytokines in PBMCs isolated from ducks immunized with rOmpA + CpG ODN were significantly higher than those of the rOmpA group. Homologous challenge result showed that the rOmpA + CpG ODN vaccine reduced the pathological score by 90% in comparison with the saline control. In conclusion, our study found that CpG ODN can enhance both humoral and cellular immunity elicited by a rOmpA vaccine. The rOmpA + CpG ODN vaccine can be further developed as a subunit vaccine against R. anatipestifer . [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
Academic Search Index |
