المؤلفون: Yue Meng, Xiaoyu Sui, Xu Pan, Ying Yang, Huimin Sui, Tao Xu, Honglian Zhang, Tingting Liu, Jicheng Liu, Pengling Ge

المصدر: Ultrasonics Sonochemistry, Vol 99, Iss , Pp 106588- (2023)

مصطلحات موضوعية: Deep eutectic solvents, Eucommia ulmoides leaves, Chlorogenic acid, Ultrasonic wave-enhanced, Macroporous adsorption resin, Chemistry, QD1-999, Acoustics. Sound, QC221-246

الوصف: This study established an integrated process for the extraction and enrichment of chlorogenic acid (CGA) from Eucommia ulmoides leaves in a deep eutectic solvent system via ultrasonic wave-enhanced adsorption and desorption practices utilizing macroporous resins. Although deep eutectic solvents (DESs) have the advantages of chemical stability, good dissolving capacity, and nonvolatilization, routine solvent recovery operations are not suitable for subsequent separation in this solvent system. Based on the above characteristics, this study integrated the extraction and enrichment processes, in which DESs extracts directly loaded onto the macroporous adsorption resin, avoiding the loss of target components in solvent recovery and redissolution processes. The screening results of solvents and resin types further showed that choline chloride-malic acid (1:1) was the optimal DES, and the NKA-II resin had high adsorption and elution performance for CGA. The viscosities of the DESs were much higher than those of water and conventional organic solvents; thus, the mass transfer resistance was large, which could also affect the adsorption behaviour of the macroporous resin. The thermal and mechanical effects of ultrasound could effectively enhance the efficiency of the mass transfer, adsorption, and desorption in the DES systems. When compared to no sonication treatment, the CGA adsorption at various ultrasonic powers (120–600 W) was examined. At optimal ethanol concentration (60%), the effect of the ultrasonic treatment on the recovery of the DESs (water eluting process) and the desorption capability of CGA were confirmed. The use of three volumes of water elution could recover the DESs without loss of CGA. The adsorption process significantly differed depending on the ultrasonic settings, and the absorption balance time and experimental adsorption capacity at equilibrium were enhanced. Additionally, the adsorption procedure of the NKA-II macroporous resin for CGA under ultrasonic treatment could be clarified by the pseudo second order kinetic equation and the Freundlich isotherm model. Thermodynamic and dynamic parameters indicated that physical adsorption was the main process of the entire procedure, and it was a spontaneous, exothermic, and entropy-reducing physical adsorption process. This study potentially indicates that the use of ultrasonication, as a high-efficiency, environmentally friendly method, can enhance the features of the macroporous resin to better purify target chemicals from a DES extract.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S1350417723003000Test; https://doaj.org/toc/1350-4177Test

الوصول الحر: https://doaj.org/article/5d06118d09074e428c42baebca2e4333Test

المؤلفون: Yue Meng, Xiaoyu Sui, Xu Pan, Xinyi Zhang, Huimin Sui, Tao Xu, Honglian Zhang, Tingting Liu, Jicheng Liu, Pengling Ge

المصدر: Ultrasonics Sonochemistry, Vol 98, Iss , Pp 106522- (2023)

مصطلحات موضوعية: Sonocrystallisation, Astragalus membranaceus var. Mongholicus roots, High-density natural deep eutectic solvents, Polysaccharides, Selective separation, Preprocessing optimization, Chemistry, QD1-999, Acoustics. Sound, QC221-246

الوصف: The water extraction and ethanol precipitation method is an extraction method based on the solubility characteristics of polysaccharides that offers wide applicability in the extraction and separation of plant polysaccharides. However, this method leads to large amounts of proteins, nucleic acids, pigments, and other impurities in the polysaccharides products, which makes downstream purification complicated and time-consuming. In this study, a green, high-density natural deep eutectic solvents was used for the high-purity extraction and separation of polysaccharides from Astragalus membranaceus (Fisch) Bge. var. Mongholicus (Bge.) Hsiao roots under ultrasound-assisted conditions. In this study, 16 different natural deep eutectic solvents were designed to screen the best solvent for extracting Astragalus polysaccharides (APSs). Based on the yield and recovery of APSs, a natural deep eutectic solvents composed of choline chloride and oxalic acid with a molar ratio of 1:2 was selected. The related factors affecting polysaccharides extraction and solvent precipitation were investigated. To improve the operating methodology, single-factor trials, a Plackett-Burman design, and a Box-Behnken design were used. The optimal extraction process conditions were obtained as follows: water content of 55%, liquid–solid ratio of 24 mL/g, ultrasonic irradiation time of 54 min, ultrasonic irradiation temperature of 50 °C, ultrasonic irradiation power of 480 W, ethanol precipitation time of 24 h, and ethanol concentration of 75%. Under optimal extraction conditions, the recovery of APSs was 61.4 ± 0.6 mg/g. Considering the special matrix characteristics of A. membranaceus var. Mongholicus roots, physical-technology-based ultrasonic waves promote penetration, and the mass transfer function also solves the bottleneck of high-viscosity deep eutectic solvents in the extraction stage. In comparison with the conventional method, the proposed method based on deep eutectic solvents isolation can significantly increase APSs recovery, which is beneficial to simplifying the process of polysaccharides purification by using solvent properties to separate extracts and reduce impurities in APSs.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S1350417723002341Test; https://doaj.org/toc/1350-4177Test

الوصول الحر: https://doaj.org/article/fef5d3a1f0074a56bd6c618ef10e30c0Test