Simple two-step covalent protein conjugation to PEG-coated nanocrystals
| العنوان: | Simple two-step covalent protein conjugation to PEG-coated nanocrystals |
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| المؤلفون: | E. E. Bila, Mateusz Banski, D. Stachowiak, O. I. Karmash, Rostyslav Bilyy, Tetiana Dumych, Artur Podhorodecki, S. Ya. Paryzhak |
| المصدر: | Ukrainian Biochemical Journal, Vol 90, Iss 2, Pp 8-12 (2018) |
| بيانات النشر: | National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine., 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Chemistry, Two step, technology, industry, and agriculture, quantum dots, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, Biochemistry, Combinatorial chemistry, 0104 chemical sciences, lcsh:Biochemistry, Nanocrystal, Simple (abstract algebra), Covalent bond, PEG ratio, PEGylated nanocomposites, lcsh:QD415-436, 0210 nano-technology, covalent protein conjugation |
| الوصف: | Covering of nanocrystals (NC) with a polyethylene glycol (PEG) envelop is a common way to increase their hydrophilicity, and compatibility with bio-systems, including increased retention time in the body. Colloidal semiconductor NC, also known as quantum dots (QD), particularly benefit from covering with PEG due to passivation of the inorganic core, while maintaining physical properties of the core. Despite many advantages of covering the surface with PEG, the covalent attachment of protein to hydroxyls of PEG is complicated. Here we propose a simple two-step approach for modification of PEG residues with subsequent covalent attachment of proteins. We were able to achieve specific NC targeting by means of attached protein as well as preserve their optical parameters (fluorescence intensity) in chemical reaction conditions. In the optimized protocol, ensuring removal of chemical byproducts by dialysis, we were able to omit the need for centrifugation (usually a limiting step due to particle size). The obtained NC-protein conjugate solutions contained 0.25x of initial unmodified NC amount, ensuring a low dilution of the sample. During all reactions the pH range was optimized to be between 6 to 8. The proposed approach can be easily modified for covalent targeting of different PEG-covered nanocomposites with proteins. |
| اللغة: | English |
| تدمد: | 2413-5003 2409-4943 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c56ad3d1a25d56629b2db340132eae43Test http://ukrbiochemjournal.org/wp-content/uploads/2018/03/Paryzhak_2_18.pdfTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c56ad3d1a25d56629b2db340132eae43 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 24135003 24094943 |
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