التفاصيل البيبلوغرافية
| العنوان: |
Effects of betulinic acid on AKT/mTOR pathway in renal cell carcinoma. |
| المؤلفون: |
Ataş, Merve Nur, Ertuğrul, Barış, İplik, Elif Sinem, Çakmakoğlu, Bedia, Ergen, Arzu |
| المصدر: |
Turkish Journal of Urology; Jan2022, Vol. 48 Issue 1, p58-63, 6p |
| مصطلحات موضوعية: |
RENAL cell carcinoma, REVERSE transcriptase polymerase chain reaction, FLOW cytometry, TERPENES, ANTINEOPLASTIC agents, MTOR inhibitors, METABOLISM, APOPTOSIS, GENE expression, BETULINIC acid, CELL proliferation, CELL lines, CASPASES, PHARMACODYNAMICS |
| Abstract (Turkish): |
Objective: Renal cancer is the most lethal among urological cancer. Treatments of renal cell carcinoma (RCC) may be possible by immune checkpoint inhibitors and drug treatment targeting different molecules. We aimed to determine the apoptotic effect of betulinic acid and its effects on expressions of apoptosis-associated genes AKT-1 and mTOR in RCC cells. Material and methods: İn this study, we investigated the apoptotic activity of betulinic acid in CAKİ-2 cell line and its effect on AKT-1 and mTOR gene expression levels. İn order to do so, following analyses were conducted: WST-1 to identify the toxic effect of betulinic acid, Caspase-3/BCA to detect caspase enzyme activity, Annexin-V and ELİSA to determine for apoptotic effect, and finally, real-time PCR for expression levels of AKT-1 and mTOR. Results: Our study showed that different concentrations of betulinic acid induced apoptosis in renal cancer; however, no effect was observed in healthy cells. İn gene expression analysis, there was statistically significant decrease in AKT-1 expression level while increasing mTOR expression level. Conclusion: We suggested that betulinic acid with its apoptotic effect on RCC line and nontoxic effect on healthy cell line and the effects on AKT/mTOR pathway may be a potential anticancer drug promising for future studies. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
