Mechanisms Underlying Regulation of Cell Cycle and Apoptosis by hnRNP B1 in Human Lung Adenocarcinoma A549 Cells
| العنوان: | Mechanisms Underlying Regulation of Cell Cycle and Apoptosis by hnRNP B1 in Human Lung Adenocarcinoma A549 Cells |
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| المؤلفون: | Juan, Han, Feng-ming, Tang, Dan, Pu, Dan, Xu, Tao, Wang, Weimin, Li |
| المصدر: | Tumori Journal. 100:102-111 |
| بيانات النشر: | SAGE Publications, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Cancer Research, Lung Neoplasms, Microscopy, Confocal, 040301 veterinary sciences, Blotting, Western, Calcium-Binding Proteins, Cell Cycle, Adenocarcinoma of Lung, Apoptosis, 04 agricultural and veterinary sciences, General Medicine, Adenocarcinoma, Real-Time Polymerase Chain Reaction, 030226 pharmacology & pharmacy, Up-Regulation, Gene Expression Regulation, Neoplastic, 0403 veterinary science, 03 medical and health sciences, 0302 clinical medicine, Microscopy, Fluorescence, Oncology, Cell Line, Tumor, Heterogeneous-Nuclear Ribonucleoprotein Group A-B, Humans, Tumor Suppressor Protein p53 |
| الوصف: | Overexpression of heterogeneous nuclear ribonucleoprotein B1 (hnRNP B1), a nuclear RNA binding protein, has been reported to occur in early-stage lung cancer and in premalignant lesions. DNA-dependent protein kinase (DNA-PK) is known to be involved in the repair of double-strand DNA breaks. Reduced capacity to repair DNA has been associated with the risk of lung cancer.We investigated a link between hnRNP B1 and DNA-PK and their effects on proliferation, cell cycle, and apoptosis in the human lung adenocarcinoma cell line A549.We found that hnRNP B1 and DNA-PK interact with each other in a complex fashion. Reducing hnRNP B1 expression in A549 cells with the use of RNAi led to upregulation of p53 activity through upregulation of DNA-PK activity but without inducing p53 expression. Further, suppression of hnRNP B1 in A549 cells slowed cell proliferation, promoted apoptosis, and induced cell cycle arrest at the G1 stage. The presence of NU7026 reduced the arrest of cells at the G1 stage and reduced the apoptosis rate while promoting cell growth.Taken together, our results demonstrate that by regulating DNA-PK activity, hnRNP B1 can affect p53-mediated cell cycle progression and apoptosis, resulting in greater cell survival and subsequent proliferation. |
| تدمد: | 2038-2529 0300-8916 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a1e5210861684458720e3c6de88aa469Test https://doi.org/10.1177/1430.15824Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....a1e5210861684458720e3c6de88aa469 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20382529 03008916 |
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