Upregulation of circulating cancer stem cell marker, DCLK1 but not Lgr5, in chemoradiotherapy-treated colorectal cancer patients
| العنوان: | Upregulation of circulating cancer stem cell marker, DCLK1 but not Lgr5, in chemoradiotherapy-treated colorectal cancer patients |
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| المؤلفون: | Afshin Abdi Rad, Gholamreza Tavoosidana, Zahra Madjd, Mohammad Sadegh Fazeli, Reza Shirkoohi, Alireza Mirzaei, Mohammad Hossein Modarressi, Masoumeh Tavakoli-Yaraki |
| المصدر: | Tumor Biology. 36:4801-4810 |
| بيانات النشر: | Springer Science and Business Media LLC, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Adult, Male, Oncology, medicine.medical_specialty, Colorectal cancer, Disease, Protein Serine-Threonine Kinases, Peripheral blood mononuclear cell, Receptors, G-Protein-Coupled, Doublecortin-Like Kinases, Cancer stem cell, Cell Line, Tumor, Internal medicine, Biomarkers, Tumor, medicine, Humans, RNA, Messenger, Stage (cooking), Pathological, Aged, Neoplasm Staging, business.industry, Intracellular Signaling Peptides and Proteins, LGR5, Chemoradiotherapy, Adjuvant, General Medicine, Middle Aged, Neoplastic Cells, Circulating, medicine.disease, Gene Expression Regulation, Neoplastic, Lymphatic Metastasis, Leukocytes, Mononuclear, Neoplastic Stem Cells, Cancer research, Female, Colorectal Neoplasms, business, Chemoradiotherapy |
| الوصف: | Cancer stem cell (CSC) markers have attracted considerable attention in tumor diagnostic, prognostic, and therapeutic implications. Detection of cancer stem cells in circulating blood using cancer stem cell markers has received remarkable attention recently. In this study, we aimed to investigate the messenger RNA (mRNA) expression level of Lgr5 and DCLK1 as most proposed colorectal CSC markers in blood circulation also determine the subsequent association to patients' clinical and pathological findings. Peripheral blood mononuclear cells (PBMCs) of 58 patients with colorectal cancer at stage I-IV with 33 out of 58 patients undergoing preoperative chemoradiotherapy (CRT), as well as 58 healthy controls have been isolated and the extracted RNAs were analyzed using real-time PCR. The mRNA expression pattern of CSC markers of patients and controls was compared using ΔΔCt method. The expression level of Lgr5 was significantly higher in colorectal cancer (CRC) patients comparing to healthy group (4.8-fold change, p |
| تدمد: | 1423-0380 1010-4283 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43cfc86ce1af99a5a78236a925ee5c3bTest https://doi.org/10.1007/s13277-015-3132-9Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....43cfc86ce1af99a5a78236a925ee5c3b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14230380 10104283 |
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