Regulation of proliferation, angiogenesis and apoptosis in hepatocellular carcinoma by miR-26b-5p
| العنوان: | Regulation of proliferation, angiogenesis and apoptosis in hepatocellular carcinoma by miR-26b-5p |
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| المؤلفون: | Bing Shao, Huizhi Sun, Yanhui Zhang, Nan Zhao, Fang Liu, Xudong Wang, Yanlei Li, Yong Wang, Baocun Sun, Jindan An, Xiulan Zhao, Qiang Gu |
| المصدر: | Tumor Biology. 37:10965-10979 |
| بيانات النشر: | Springer Science and Business Media LLC, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Adult, Male, 0301 basic medicine, Pathology, medicine.medical_specialty, Carcinoma, Hepatocellular, MMP2, Angiogenesis, Blotting, Western, Mice, Nude, Apoptosis, Biology, medicine.disease_cause, Polymerase Chain Reaction, 03 medical and health sciences, 0302 clinical medicine, microRNA, medicine, Animals, Humans, Viability assay, Aged, Cell Proliferation, Oligonucleotide Array Sequence Analysis, Tube formation, Mice, Inbred BALB C, Neovascularization, Pathologic, Cell growth, Liver Neoplasms, General Medicine, Middle Aged, Immunohistochemistry, digestive system diseases, Gene Expression Regulation, Neoplastic, MicroRNAs, 030104 developmental biology, Cell culture, 030220 oncology & carcinogenesis, Cancer research, Heterografts, Female, Transcriptome, Carcinogenesis |
| الوصف: | MicroRNAs (miRNAs) play vital roles in cell proliferation, differentiation and apoptosis in hepatocellular carcinoma (HCC). miR-26b has been confirmed as an important regulator in carcinogenesis and other pathological processes. miR-26b-5p is one member of the mature miR-26 family, and its functional role in proliferation, angiogenesis and apoptosis in HCC remains unknown. Here, we demonstrate that miR-26b-5p expression was significantly decreased in HCC tissues and HCC cell lines compared with normal liver tissues and liver cells by quantitative real-time polymerase chain reaction (qRT-PCR). The relationships between miR-26b-5p and the clinical characteristics of HCC patients were further analysed, and miR-26b-5p was positively correlated with the differentiation of HCC cells. Computational searches were further used to identify the downstream targets and signalling pathways of miR-26b-5p in HCC cells. Cell viability, proliferation and tube formation abilities were assessed by scrape, 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and three-dimensional culture assays to confirm that miR-26b-5p inhibited HCC cell growth and impaired the tube formation ability of the HCC cells. Both in vitro and in vivo studies showed that miR-26b-5p could suppress vascular mimicry (VM) and angiogenesis by down-regulating the expression of VE-cadherin, Snail and MMP2 and could inhibit the apoptosis of HCC cells. Using mouse models, we revealed that tumours derived from miR-26b-5p-expressing HCC cells displayed a significant decrease in microvessel density compared with those derived from control cells. Therefore, our data provide further insight into the role of miR-26b-5p as a negative regulator of proliferation, angiogenesis, and apoptosis in HCC. |
| تدمد: | 1423-0380 1010-4283 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e117bbe84dc0ebc57ba018af7e165f4eTest https://doi.org/10.1007/s13277-016-4964-7Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e117bbe84dc0ebc57ba018af7e165f4e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14230380 10104283 |
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