MicroRNA-7 downregulates the oncogene VDAC1 to influence hepatocellular carcinoma proliferation and metastasis
| العنوان: | MicroRNA-7 downregulates the oncogene VDAC1 to influence hepatocellular carcinoma proliferation and metastasis |
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| المؤلفون: | Lei Yin, Yong Qiang, Jiahui Chen, Yinda Wang, Lirong Zhu, Feiran Wang, Zhong Chen, Xian Shao, Yasu Jiang |
| المصدر: | Tumor Biology. 37:10235-10246 |
| بيانات النشر: | Springer Science and Business Media LLC, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Adult, Male, 0301 basic medicine, Carcinoma, Hepatocellular, Blotting, Western, Down-Regulation, Kaplan-Meier Estimate, Biology, Real-Time Polymerase Chain Reaction, medicine.disease_cause, Metastasis, 03 medical and health sciences, 0302 clinical medicine, microRNA, Biomarkers, Tumor, medicine, Humans, Neoplasm Invasiveness, Aged, Cell Proliferation, Retrospective Studies, Oncogene, Voltage-Dependent Anion Channel 1, Liver Neoplasms, General Medicine, Middle Aged, Prognosis, medicine.disease, Immunohistochemistry, digestive system diseases, Gene Expression Regulation, Neoplastic, MicroRNAs, 030104 developmental biology, Real-time polymerase chain reaction, Tumor progression, 030220 oncology & carcinogenesis, Hepatocellular carcinoma, Cancer research, Female, Carcinogenesis |
| الوصف: | Recent studies have been shown that voltage-dependent anion channel 1 (VDAC1) plays an important role in carcinogenesis. However, its molecular biological function in hepatocellular carcinoma (HCC) has not been entirely clarified. This study investigated the expression of VDAC1 in HCC and its prognostic value for HCC patients. Furthermore, we also identify the relevant VDAC1 direct target. Western blot, real-time quantitative PCR (qRT-PCR), and immunohistochemical (IHC) staining were performed to detect the expression of VDAC1 in HCC. Furthermore, the relationship between the VDAC1 level and clinicopathological features and prognostic values was explored. The effects of VDAC1 on HCC cell proliferation, migration, and invasion were also investigated in vitro. Predicted target gene of VDAC1 was determined by dual-luciferase reporter assay, qRT-PCR, and Western blot analyses. Our results revealed elevated VDAC1 messenger RNA (mRNA) (P = 0.0020) and protein (P = 0.0035) expression in tumor tissue samples compared with paired adjacent non-tumorous tissue samples. High VDAC1 expression was correlated with distant metastasis (P = 0.025), differentiation (P = 0.002), and advanced tumor stage (P = 0.004) in HCC patients. Kaplan-Meier survival analysis demonstrated that high expression of VDAC1 was significantly correlated with a poor prognosis for HCC patients (P |
| تدمد: | 1423-0380 1010-4283 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ae59f817e965e0b60fcd04af800f2f9Test https://doi.org/10.1007/s13277-016-4836-1Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2ae59f817e965e0b60fcd04af800f2f9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14230380 10104283 |
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