Curcuma longa Linn extract suppresses neuronal apoptosis induction by sevoflurane via activation of the ERK1/2 pathway
| العنوان: | Curcuma longa Linn extract suppresses neuronal apoptosis induction by sevoflurane via activation of the ERK1/2 pathway |
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| المؤلفون: | Yu, Zhou, Yan, Yao, Lou, Ying |
| المصدر: | Tropical Journal of Pharmaceutical Research; Vol. 20 No. 2 (2021); 269-274 |
| بيانات النشر: | African Journals Online (AJOL), 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Pharmaceutical Science, Pharmacology (medical), Anesthesia, Neurotoxicity, Sphingomyelin, Apoptosis, Curcuma longa |
| الوصف: | Purpose: To investigate Curcuma longa Linn against neuronal damage induced by exposure to sevoflurane during surgical procedures. Methods: A sealed box made of transparent glass was used for anaesthetic exposure of neurons. The neurons were exposed to Curcuma longa Linn at doses of 1.5, 3, 6 and 12 μM prior to viability assessment using MTT assay. The effect of Curcuma longa Linn treatment on protein expression was determined using western blotting. Results: Sevoflurane exposure led to significant and time-dependent reductions in neuronal proliferation, when compared to unexposed cells (p < 0.05). Curcuma longa Linn at doses of 1.5, 3, 6 and 12 μM significantly decreased sevoflurane-mediated neuronal apoptosis. It reduced cleaved caspase-3 and Bax levels in neurons. However, the Curcuma longa Linn-mediated inhibition of sevoflurane-induced neuronal apoptosis was significantly suppressed by VPC23019 (p < 0.05). The p- ERK1/2 level was dose-dependently up-regulated in neurons exposed to sevoflurane on treatment with Curcuma longa Linn. Moreover, VPC23019 reversed the upregulatory effect of Curcuma longa Linn on p-ERK1/2 expression in sevoflurane-exposed neurons (p < 0.05). Conclusion: Curcuma longa Linn reversed sevoflurane-induced neuronal apoptosis by elevating p- ERK1/2 expression. Therefore, Curcuma longa Linn exerts inhibitory effect on anaesthesia-induced apoptosis in neurons, and may be useful for the treatment of this condition. |
| وصف الملف: | application/pdf |
| تدمد: | 1596-9827 1596-5996 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::580720afa48548b0c2527bdab388c9e2Test https://doi.org/10.4314/tjpr.v20i2.7Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....580720afa48548b0c2527bdab388c9e2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15969827 15965996 |
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