دورية أكاديمية
Investigation of murine spleen as a niche for hematopoiesis
| العنوان: | Investigation of murine spleen as a niche for hematopoiesis |
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| المؤلفون: | Tan, Jonathan, O'Neill, Helen |
| المصدر: | Transplantation |
| بيانات النشر: | Lippincott Williams & Wilkins |
| المجموعة: | Australian National University: ANU Digital Collections |
| مصطلحات موضوعية: | Keywords: blood group antigen, allotype, animal experiment, animal model, animal tissue, antigen presenting cell, article, B lymphocyte, bone marrow cell, circulation, controlled study, dendritic cell, engraftment, hematopoiesis, hematopoietic stem cell, host cell, Hematopoietic stem cells, Myelopoiesis., Spleen |
| الوصف: | Background. Spleen as a lymphoid tissue is specialized for monitoring blood and mounting immunity against blood-borne antigens. Antigen-presenting cells present in spleen commonly develop from bone marrow-derived precursors that enter blood circulation. However, a distinct splenic myeloid antigen-presenting cell subset described in this laboratory, namely "dendritic-like cells" (L-DC), has been hypothesized not to share a bone marrow origin. Methods. In this study, the presence of endogenous hematopoietic progenitors in spleen was investigated by transplanting intact spleen into allotype-distinct recipients and monitoring development of progeny cells in grafted tissues. Results. Successful engraftment of donor spleens was achieved for up to 4 weeks. After 2 weeks, donor-type myeloid cells, dendritic cells (DC) and few B cells were observed in spleen grafts indicative of spleen-endogenous hematopoiesis. An influx of host-type hematopoietic stem cell, as well as host-type lymphoid and myeloid cells, was also observed. Conclusion. Evidence for the maintenance of donor-type myeloid cells after 2 to 4 weeks reflected development from hematopoietic progenitors endogenous to spleen. L-DC were also detected in spleen grafts; however, within the graft microenvironment they were one of the several DC and myeloid subsets, including activated DC, resembling L-DC. Overall, this study adds further evidence that spleen can support endogenous myelopoiesis. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 0041-1337 |
| العلاقة: | http://hdl.handle.net/1885/34662Test; https://openresearch-repository.anu.edu.au/bitstream/1885/34662/5/Publ_Investigation_of_Murine_Spleen_Tan_ONeill.pdf.jpgTest; https://openresearch-repository.anu.edu.au/bitstream/1885/34662/7/01_Tan_Investigation_of_murine_spleen_2010.pdf.jpgTest |
| DOI: | 10.1097/TP.0b013e3181c42f70 |
| الإتاحة: | https://doi.org/10.1097/TP.0b013e3181c42f70Test http://hdl.handle.net/1885/34662Test https://openresearch-repository.anu.edu.au/bitstream/1885/34662/5/Publ_Investigation_of_Murine_Spleen_Tan_ONeill.pdf.jpgTest https://openresearch-repository.anu.edu.au/bitstream/1885/34662/7/01_Tan_Investigation_of_murine_spleen_2010.pdf.jpgTest |
| رقم الانضمام: | edsbas.3B5EEB24 |
| قاعدة البيانات: | BASE |
| تدمد: | 00411337 |
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| DOI: | 10.1097/TP.0b013e3181c42f70 |