التفاصيل البيبلوغرافية
| العنوان: |
Modification of urinary N7-methylguanine excretion in smokers by glutathione-S-transferase M1 polymorphism |
| المؤلفون: |
Lin, I-Hsin1, Chao, Mu-Rong2, Hu, Chiung-Wen1, Ho, Ming-Lin3, Huang, Jo-Yun1, Lee, Hong-Shen1, Chen, Chun-Chieh4, Wong, Ruey-Hong1 rueyhong@csmu.edu.tw |
| المصدر: |
Toxicology. Jun2009, Vol. 260 Issue 1-3, p1-6. 6p. |
| مصطلحات موضوعية: |
*PHYSIOLOGICAL effects of tobacco, *GLUTATHIONE transferase, *GENETIC polymorphisms, *DNA adducts, *METHYLATION, *TOBACCO smoke, *URINALYSIS, *CARCINOGENS, *CREATININE, *TANDEM mass spectrometry |
| مستخلص: |
Abstract: Tobacco smoke contains many carcinogens which can lead to DNA methylation. Glutathione-S-transferases (GSTs) are detoxifying enzymes, and the effects of the genes GSTM1, GSTT1, and GSTP1 on cigarette smoke-induced methylated adducts have not been elucidated. Therefore, we investigated the association of the GSTM1, GSTT1, and GSTP1 genes and N7-methylguanine (N7-MeG) adducts in smokers. Urinary N7-MeG concentrations were measured by using liquid chromatography–tandem mass spectrometry in 112 smokers and 89 non-smokers, and GSTM1, GSTT1, and GSTP1 genotypes were identified by polymerase chain reaction. Smokers had higher N7-MeG concentrations than did non-smokers (3238±305ng/mg creatinine [standard error] vs. 2386±153ng/mg creatinine; P =0.01). Higher N7-MeG concentrations were observed with the GSTM1 null genotype than with the GSTM1 non-null genotype (3230±292ng/mg creatinine vs. 2336±153ng/mg creatinine; P =0.007), particularly in smokers (3775±483ng/mg creatinine vs. 2468±228ng/mg creatinine; P =0.02). However, the GSTT1 and GSTP1 genotypes were not associated with urinary N7-MeG concentrations. Therefore, the susceptible GSTM1 genotype may modulate the concentrations of N7-MeG adducts in the DNA of smokers. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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