Reduction of glutamatergic neurotransmission by prolonged exposure to dieldrin involves NMDA receptor internalization and metabotropic glutamate receptor 5 downregulation
| العنوان: | Reduction of glutamatergic neurotransmission by prolonged exposure to dieldrin involves NMDA receptor internalization and metabotropic glutamate receptor 5 downregulation |
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| المؤلفون: | Mireia Galofré, Cristina Suñol, Víctor Briz |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| بيانات النشر: | Oxford University Press, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Time Factors, Cell Survival, Receptor, Metabotropic Glutamate 5, Excitotoxicity, Down-Regulation, Glutamic Acid, Gestational Age, Biology, Pharmacology, Receptors, Metabotropic Glutamate, Toxicology, medicine.disease_cause, Receptors, N-Methyl-D-Aspartate, Synaptic Transmission, GABA Antagonists, Mice, Glutamatergic, Dieldrin, chemistry.chemical_compound, Pregnancy, medicine, Animals, GABA-A Receptor Antagonists, Pesticides, Receptor, Cells, Cultured, Cerebral Cortex, Neurons, Dose-Response Relationship, Drug, L-Lactate Dehydrogenase, Metabotropic glutamate receptor 5, Glutamate receptor, Neurotoxicity, Receptors, GABA-A, medicine.disease, chemistry, nervous system, NMDA receptor, Calcium, Female |
| الوصف: | Dieldrin was previously used as a pesticide. Although its use has been discontinued, humans are still exposed to it due to its high environmental persistence and because it accumulates in the adipose tissue of animals. Acute exposure to dieldrin provokes convulsions due to its antagonism on the gamma-aminobutyric acid-A (GABAA) receptor. However, little is known about the effects of low chronic exposure to this pollutant. In the present work, we use primary cultures of cortical neurons to study the mechanisms involved in the toxic action of dieldrin. We found that 2 and 6 days in vitro (DIV) exposure to a subcytotoxic concentration (60nM) of dieldrin reduced the increase in intracellular calcium concentration ([Ca2+]i) and the excitotoxicity caused by glutamate. Exposure to dieldrin for 6 DIV induced N-methyl-D-aspartate receptor (NMDAR) internalization and reduced metabotropic glutamate receptor 5 (mGLUR5) levels. Double immunostaining for NMDAR and mGLUR5 showed that these receptors lose colocalization on the cell membrane in neurons treated with dieldrin. No changes were observed in receptor functionalities or receptor levels after 2 DIV of exposure to dieldrin. However, the increase in [Ca2+]i induced by coactivation of NMDAR and mGLUR5 was significantly reduced. Thus, a functional interaction between the two receptors seems to play an important role in glutamate-induced excitotoxicity. We confirm that permanent blockade of the GABAA receptor by this persistent pesticide triggers adaptive neuronal changes consisting of a reduction of glutamatergic neurotransmission. This might explain the cognitive and learning deficits observed in animals after chronic treatment with dieldrin. © The Author 2009. Published by Oxford University Press on behalf of the Society of Toxicology. This work was funded by the Instituto de Salud Carlos III, Ministry of Health, Spain (grant number PI 061212); Generalitat de Catalunya (grant number 2005/SGR/00826). V.B. is recipient of a predoctoral fellowship from Institut d’Investigacions Biomèdiques August Pi i Sunyer. |
| اللغة: | English |
| تدمد: | 1096-0929 1096-6080 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6cec7e7978bb1e814b290bf75e5527faTest http://hdl.handle.net/10261/76050Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6cec7e7978bb1e814b290bf75e5527fa |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10960929 10966080 |
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