Cis-Diamminedichloroplatinum (II)-induced Acute Renal Failure in the Rat: Enzyme Histochemical Studies
| العنوان: | Cis-Diamminedichloroplatinum (II)-induced Acute Renal Failure in the Rat: Enzyme Histochemical Studies |
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| المؤلفون: | Benjamin F. Trump, Shreekant Chopra, James S. Kaufman, Thomas W. Jones, Walter Flamenbaum |
| المصدر: | Toxicologic Pathology. 13:296-305 |
| بيانات النشر: | SAGE Publications, 1985. |
| سنة النشر: | 1985 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, medicine.medical_specialty, ATPase, Acid Phosphatase, Glycerolphosphate Dehydrogenase, Dehydrogenase, Toxicology, Pathology and Forensic Medicine, 03 medical and health sciences, Internal medicine, medicine, Animals, Gamma-glutamyltransferase, Molecular Biology, chemistry.chemical_classification, L-Lactate Dehydrogenase, 030102 biochemistry & molecular biology, biology, Histocytochemistry, Succinate dehydrogenase, Acid phosphatase, Acute kidney injury, Rats, Inbred Strains, gamma-Glutamyltransferase, Cell Biology, Acute Kidney Injury, Alkaline Phosphatase, medicine.disease, Rats, Succinate Dehydrogenase, Kidney Tubules, 030104 developmental biology, Enzyme, Endocrinology, chemistry, Glucose-6-Phosphatase, biology.protein, Alkaline phosphatase, Cisplatin, Sodium-Potassium-Exchanging ATPase |
| الوصف: | Enzyme histochemical techniques were utilized to examine the progression and extent of proximal tubular injury during the development of cis-diamminedichloroplatinum (II) (CDDP)-induced acute renal failure. Acute renal failure was induced in male rats by the intraperitoneal administration of 10 mg CDDP/kg body weight. At 6, 24, 48, 72, and 96 hr following treatment, renal function was assessed and tissue was collected for renal morphologic and enzyme histochemical studies. The enzymes examined were γ-glutamyl transpeptidase, alkaline phosphatase, sodium-potassium ATPase (nitrophenyl phosphatase), acid phosphatase, glucose-6-phosphatase, succinic dehydrogenase, α-glycerophosphate dehydrogenase, and lactic dehydrogenase. By 24 hr, the activity of acid phosphatase was reduced throughout the proximal tubule, with the greatest decrease occurring in the P3 segment of the proximal tubule located in the outer stripe of the outer medulla. Changes in the histochemical staining of the remaining enzymes were not consistently observed until 48 or, in some cases, 72 hr. These alterations involved all portions of the proximal tubule with the most severe changes involving P3. The results of the enzyme histochemical studies along with the morphologic findings indicating that the initiation of CDDP-induced acute renal failure, first apparent at 48 hr in this model, is associated with cell injury throughout the proximal tubule. The majority of the histochemical changes did not become apparent until late in the course of tubular injury. This suggests that most of the changes in enzyme activity represent nonspecific effects of CDDP-induced tubular injury, as opposed to direct enzyme inhibition by the drug. |
| تدمد: | 1533-1601 0192-6233 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6120362fefc631da53801d88209203e1Test https://doi.org/10.1177/019262338501300406Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....6120362fefc631da53801d88209203e1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15331601 01926233 |
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