Differential Effects of Bone Morphogenetic Protein-2 and Transforming Growth Factor-β1 on Gene Expression of Collagen-Modifying Enzymes in Human Adipose Tissue–Derived Mesenchymal Stem Cells
| العنوان: | Differential Effects of Bone Morphogenetic Protein-2 and Transforming Growth Factor-β1 on Gene Expression of Collagen-Modifying Enzymes in Human Adipose Tissue–Derived Mesenchymal Stem Cells |
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| المؤلفون: | Jenneke Klein-Nulend, Marlene Knippenberg, Paul I. J. M. Wuisman, Ruud A. Bank, Marco N. Helder, Behrouz Zandieh Doulabi |
| المساهمون: | Orale Celbiologie (OUD, ACTA), Parodontologie (OUD, ACTA), Maxillofacial Surgery (AMC + VUmc), Oral Cell Biology, Academic Centre for Dentistry Amsterdam, TNO Kwaliteit van Leven, Molecular cell biology and Immunology, Orthopedic Surgery and Sports Medicine, MOVE Research Institute |
| المصدر: | Tissue Engineering. Part A, 15(8), 2213-2225. Mary Ann Liebert Inc. Knippenberg, M, Helder, M N, Doulabi, B Z, Bank, R A, Wuisman, P I J M & Klein-Nulend, J 2009, ' Differential effects of bone morphogenetic protein-2 and transforming growth factor-beta1 on gene expression of collagen-modifying enzymes in human adipose tissue-derived mesenchymal stem cells ', Tissue Engineering Part A, vol. 15, no. 8, pp. 2213-2225 . https://doi.org/10.1089/ten.tea.2007.0184Test Tissue Engineering-Part A. Mary Ann Liebert Inc. Knippenberg, M, Helder, M N, Doulabi, B Z, Bank, R A, Wuisman, P I J M & Klein-Nulend, J 2009, ' Differential effects of bone morphogenetic protein-2 and transforming growth factor-beta 1 on gene expression of collagen-modifying enzymes in human adipose tissue-derived mesenchymal stem cells ', Tissue Engineering. Part A, vol. 15, no. 8, pp. 2213-2225 . https://doi.org/10.1089/ten.tea.2007.0184Test Tissue Engineering Part A, 15(8), 2213-2225. Mary Ann Liebert Inc. Knippenberg, M, Helder, M N, Doulabi, B Z, Bank, R A, Wuisman, P I J M & Klein-Nulend, J 2009, ' Differential effects of bone morphogenetic protein-2 and transforming growth factor-β1 on gene expression of collagen-modifying enzymes in human adipose tissue-derived mesenchymal stem cells ', Tissue Engineering-Part A . https://doi.org/10.1089/ten.tea.2007.0184Test Tissue Engineering-Part A, 8, 15, 2213-2225 |
| بيانات النشر: | Mary Ann Liebert Inc, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Biomedical Research, Bone tissue, medicine.medical_treatment, Bone Morphogenetic Protein 2, Adipose tissue, Mechanical properties, Core Binding Factor Alpha 1 Subunit, Bioactivity, Biochemistry, Bone tissue engineering, Protein-Lysine 6-Oxidase, Extracellular matrix, Physical and mechanical properties, Osteogenic differentiation, Biomechanics, Enzyme activity, Extracellular matrices, Mesenchymal stem cell, Catalysts, Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase, Growth factor, Enzymes, Cell biology, Enzyme inhibition, medicine.anatomical_structure, Flowcharting, Adipose Tissue, Transforming growth factors, Alkaline phosphatase activity, Female, Collagen, Procollagen, Type I collagen, Adult, medicine.medical_specialty, Adolescent, Cells, Biomedical Engineering, Bioengineering, Lysyl oxidase, Biology, Bone morphogenetic protein 2, Collagen Type I, Gene Expression Regulation, Enzymologic, Transforming Growth Factor beta1, Biomaterials, Internal medicine, Hydroxylases, medicine, Humans, Tissue engineering, Bone, Mesenchymal Stem Cells, Alkaline Phosphatase, Human adipose tissue, Procollagen peptidase, Endocrinology, Differential effect, Osteopontin, Bone morphogenetic protein-2, Cell culture, Gene expression, Peptides, Post-translational modifications |
| الوصف: | Adipose tissue-derived mesenchymal stem cells (AT-MSCs) in combination with bone morphogenetic protein-2 (BMP-2) or transforming growth factor-beta1 (TGF-beta1) are under evaluation for bone tissue engineering. Posttranslational modification of type I collagen is essential for functional bone tissue with adequate physical and mechanical properties. We investigated whether BMP-2 (10-100 ng/mL) and/or TGF-beta1 (1-10 ng/mL) affect gene expression of alpha2(I) procollagen and collagen-modifying enzymes, that is, lysyl oxidase and lysyl hydroxylases 1, 2, and 3 (encoded by PLOD1, 2, and 3), by human AT-MSCs. BMP-2, but not TGF-beta1, increased alkaline phosphatase activity after 28 days, indicating osteogenic differentiation of AT-MSCs. At day 4, both BMP-2 and TGF-beta1 upregulated alpha2(I) procollagen and PLOD1, which was downregulated at day 28. TGF-beta1, but not BMP-2, downregulated PLOD3 at day 28. Lysyl oxidase was upregulated by TGF-beta1 at day 4 and by BMP-2 at day 7. Neither BMP-2 nor TGF-beta1 affected PLOD2. In conclusion, these results suggest that AT-MSCs differentially respond to BMP-2 and TGF-beta1 with changes in gene expression of collagen-modifying enzymes. AT-MSCs may thus be able to appropriately modify type I collagen to form a functional bone extracellular matrix for tissue engineering, dependent on the growth factor added. |
| تدمد: | 1937-335X 1937-3341 2152-4947 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a0e3a8fc0ea5fa47142d901e8ef3ff3Test https://doi.org/10.1089/ten.tea.2007.0184Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7a0e3a8fc0ea5fa47142d901e8ef3ff3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1937335X 19373341 21524947 |
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