التفاصيل البيبلوغرافية
| العنوان: |
Tectorigenin protect HUVECs from H2O2-induced oxidative stress injury by regulating PI3K/Akt pathway. |
| المؤلفون: |
Chen, Xiao, Zhang, Weijia, Sun, Lirui, Lian, Yonghong |
| المصدر: |
Tissue & Cell; Feb2021, Vol. 68, pN.PAG-N.PAG, 1p |
| مصطلحات موضوعية: |
OXIDATIVE stress, GLUTATHIONE peroxidase, LACTATE dehydrogenase, SUPEROXIDE dismutase, ENDOTHELIAL cells, UMBILICAL veins |
| مستخلص: |
• H 2 O 2 decreased the viability of HUVECs. • H 2 O 2 increased the leakage of LDH form HUVECs. • Tectorigenin decreased oxidative stress injury in H 2 O 2 -induced HUVECs. • Tectorigenin upregulated the PI3K/Akt pathway activation in H 2 O 2 -induced HUVECs. Oxidative stress injury (OSI) occurs in many cardiovascular diseases, and the OSI of endothelial cells is the main pathological basis of these diseases. Tectorigenin has an effect on oxidative stress in fibroblasts, keratinocytes, and neuroblastoma. This study attempted to reveal the effect of Tectorigenin on OSI in endothelial cells. An OSI cell model was firstly established by treating human umbilical vein endothelial cells (HUVECs) with H 2 O 2. The H 2 O 2 -induced HUVECs were further pre-treated with Tectorigenin or PI3K inhibitor. Then the viability and apoptosis of HUVECs were evaluated using MTT, Hochest 33258 staining and TUNEL staining. Lactate dehydrogenase (LDH) leakage, enzyme activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) level were measured through colorimetric assays. The expressions of apoptosis-related factors and the activation of the PI3K/Akt pathway in HUVECs were detected by RT-qPCR or Western blot. Tectorigenin had no inhibiting effect on the viability of HUVECs at the concentrations of 0.1, 0.5, 0.5, 1, and 10 μmol/L. Tectorigenin reversed the H 2 O 2 induced-destruction of HUVECs morphology. Tectorigenin increased the viability and decreased the apoptosis of H 2 O 2 -induced HUVECs. Tectorigenin increased Bcl-2 expression and the enzyme activities of SOD and GSH-Px, but decreased LDH leakage, MDA level, and the expressions of Bax and Cleaved Caspase-3 in H 2 O 2 -induced HUVECs. Furthermore, Tectorigenin increased the ratios of p-PI3K to PI3K and p-Akt to Akt in H 2 O 2 -induced HUVECs. PI3K inhibitor had an opposite effect of Tectorigenin on the OSI in H 2 O 2 -induced HUVECs and its effect was further reversed by Tectorigenin. Tectorigenin protected HUVECs against H 2 O 2 -induced OSI via PI3K/Akt pathway. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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