Proinflammatory properties of murine aortic endothelial cells exclusively expressing a non cleavable form of TNFα
| العنوان: | Proinflammatory properties of murine aortic endothelial cells exclusively expressing a non cleavable form of TNFα |
|---|---|
| المؤلفون: | Matthias Canault, Christoph Mueller, Bernadette Bonardo, Denis Bernot, Franck Peiretti, Gilles Nalbone, Irène Juhan-Vague, Paule Deprez |
| المصدر: | Thrombosis and Haemostasis. 92:1428-1437 |
| بيانات النشر: | Georg Thieme Verlag KG, 2004. |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | Lipopolysaccharides, Chemokine, Time Factors, Endothelium, Receptors, CCR2, medicine.medical_treatment, Down-Regulation, Enzyme-Linked Immunosorbent Assay, Mice, Transgenic, Proinflammatory cytokine, Mice, medicine, Animals, Receptors, Tumor Necrosis Factor, Type II, RNA, Messenger, Transgenes, Receptor, Chemokine CCL5, Aorta, Cells, Cultured, Inflammation, biology, Interleukin-6, Reverse Transcriptase Polymerase Chain Reaction, Tumor Necrosis Factor-alpha, business.industry, Macrophages, Thrombosis, Hematology, Flow Cytometry, Intercellular Adhesion Molecule-1, Molecular biology, Culture Media, Endothelial stem cell, medicine.anatomical_structure, Cytokine, Immunology, Macrophages, Peritoneal, biology.protein, Receptors, Chemokine, Tumor necrosis factor alpha, Endothelium, Vascular, Tumor necrosis factor receptor 2, business |
| الوصف: | SummarySoluble (sTNF) and transmembrane (tmTNF) forms of TNFα (TNF) have distinct proinflammatory effects. We investigated whether tmTNF altered the synthesis of some proinflammatory proteins involved in atherothrombosis, in murine aortas and aortic endothelial cells (MAEC). Samples were obtained from wild-type (WT) mice and TNF-deficient mice that express a mutated non cleavable tmTNF transgene (tmTNFnc).The levels of secreted MCP-1, RANTES, IL-6, PAI-1, soluble ICAM-1, and soluble TNF receptor type 1 (TNFR1; CD120a) antigens, MMP-9 activity and of cell surface ICAM-1 were not significantly different between the two types of MAEC.The magnitude of endotoxin-stimulated production of RANTES, MCP-1 and IL-6 was similar in the two types of cells. Of note, the amount of synthesized TNF receptor type 2 (TNFR2; CD120b), measured by its secreted (in aorta and MAEC), intracellular and mRNA levels (in MAEC), was significantly 4-fold lower in tmTNFnc than in WT mice, both in basal and endotoxin-stimulated conditions. A neutralizing anti-TNF antibody or the recombinant murine TNF did not modify the magnitude of the difference in TNFR2 production between the two types of cells, suggesting a preponderant role of tmTNF in the down-regulation of TNFR2 synthesis. Macrophages of tmTNFnc mice also produced less TNFR2 than WT macrophages (−30%). Plasmas of tmTNFnc mice contained significantly less sTNFR2 than WT mice (−75%). In conclusion, an increase in tmTNF levels, rather than the lack of sTNF, significantly down-modulated TNFR2 synthesis in aortic endothelial cells, but had no major influence on the synthesis of some major pro-inflammatory and pro-atherothrombotic proteins. |
| تدمد: | 2567-689X 0340-6245 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b370687c944d3019f9309748369ebd5Test https://doi.org/10.1160/th04-06-0344Test |
| رقم الانضمام: | edsair.doi.dedup.....7b370687c944d3019f9309748369ebd5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2567689X 03406245 |
|---|