التفاصيل البيبلوغرافية
| العنوان: |
Heart, mitochondria and ischemia reperfusion injuries : impact of diabetes and opioids postconditioning ; Coeur, mitochondries, lésions d'ischémie-reperfusion : impact du diabète et du post-conditionnement par les ligands opiacés |
| المؤلفون: |
Richer, Romain |
| المساهمون: |
Normandie, Allouche, Stéphane, Coulbault, Laurent |
| المصدر: |
Theses.fr |
| سنة النشر: |
2018 |
| مصطلحات موضوعية: |
Infarctus, Heart, Infarction, Mitochondria, Diabetes mellitus, Cardioprotective agent, Opioids, envir, socio |
| الوصف: |
The morbidity and the mortality of the heart stroke have been reduced over the last 30 years and it was related to an improvement of patient care. The early reperfusion of the heart is an essential step, but is responsible for ischemia-reperfusion injuries. Both ischemic and pharmacological conditionings were shown to increase heart function, but these beneficial effects are reduced in a context of diabetes mellitus.First, we studied the effect of type 2 diabetes mellitus on mitochondrial function. Studies were performed on heart trabeculae, and in subsarcolemmal (SSM) or interfibrillar (IFM) mitochondria extracted from human atrial appendages using polarographic, spectrophotometric and proteomic analyses. Whereas differences on enzymatic activities and in protein expression were observed between SSM and IFM, we did not find any deleterious effect of diabetes mellitus on mitochondrial function. Second, using a Langendorff’s apparatus, we developed an experimental model in mouse to study ischemia-reperfusion injuries. Pharmacological post-conditioning was tested by using various opioid agonists and antagonists, including morphine, [D-Pen2,D-Pen5]-enkephalin (DPDPE), naloxone, and naltrindole. The effects were observed on heart function and the volume of necrosis. All treatments were effective to reduce the necrosis in the heart compared to control condition. After 60 minutes of reperfusion, cardiac function was also improved with morphine, naloxone, and the association of morphine-naloxone, and DPDPE-naltrindole. A better understanding of the molecular mechanisms is needed to improve pharmacological post-conditioning in patients, particularly in diabetics, presenting with heart stroke. ; Depuis plus de 30 ans, les progrès thérapeutiques ont permis de réduire la morbi-mortalité liée à l’infarctus du myocarde. La reperfusion est une étape essentielle dans la prise en charge des patients, mais est également à l’origine de lésions tissulaires cardiaques. Plusieurs travaux ont montré que le conditionnement ischémique ... |
| نوع الوثيقة: |
thesis |
| اللغة: |
French |
| العلاقة: |
10670/1.5vu47b; http://www.theses.fr/2018NORMC408Test |
| الإتاحة: |
http://www.theses.fr/2018NORMC408Test |
| حقوق: |
other |
| رقم الانضمام: |
edsbas.71849EFF |
| قاعدة البيانات: |
BASE |
