التفاصيل البيبلوغرافية
| العنوان: |
Immunogenicity of cardiac progenitors derived from embryonic stem cells ; Etude de l’immunogénicité des progéniteurs cardiaques dérivés des cellules souches embryonnaires |
| المؤلفون: |
Calderon, Damelys |
| المساهمون: |
Paris 5, Menasché, Philippe, Chatenoud, Lucienne |
| المصدر: |
Theses.fr |
| سنة النشر: |
2013 |
| مصطلحات موضوعية: |
Immunogénicité, Cellules souches embryonnaires, Anticorps anti-CD3, Cellules souches mésenchymateuses, Bioluminescence, Immunogenicity, Embryonic stem cells, CD3 antibody, Mesenchymal stem cells, envir, socio |
| الوصف: |
The present work concerned the analysis of the immunogenicity of cardiac progenitors derived from human embryonic stem cells. Our aim was to understand the cellular and molecular mechanisms which underlie this immunogenicity and to surmount it by setting up strategies of immune-intervention. The study consisted in two major components, one in vitro and the other in vivo using experimental mice models. The in vitro analyses were assessed by the mixed leukocyte reaction method, where human cardiac progenitors were cultured with allogeneic lymphocytes. The results showed that the cardiac progenitors are indeed immunogenic and that the immune response that they induce could be modulated by mesenchymal stromal cells derived from adipose tissue. Moreover, we confirmed the expression of class I histocompatibility molecules on the surface of cardiac progenitors, an expression which seems modulated during the culture. The in vivo models that we used consisted of the grafting of embryoïdes bodies and cardiac progenitors derived from mouse embryonic stem cells in an allogeneic context. Cells were grafted in different sites of immunocompetent mice (myocardium, renal capsule, muscle gastrocnemius). The results showed highlighted that at the same time both embryoïdes bodies and cardiac progenitors are rejected among untreated immunocompetents hosts, whereas their survival is extended by anti-CD3 treatments, In addition, anti-CD3 treatment prolongs the survival of grafted cells, either by immunosuppression or by inducing immune tolerance according to the timing when it is applied. ; Le sujet de ce travail de thèse a concerné l'analyse de l’immunogénicité de progéniteurs cardiaques issus de cellules souches embryonnaires humaines. Le but a été double. D’une part, comprendre les mécanismes cellulaires et moléculaires qui sous-tendent cette immunogénicité et, d’autre part, mettre en place des stratégies d’immuno-intervention permettant de la surmonter.Le travail a comporté deux volets, l’un in vitro et l’autre in vivo utilisant ... |
| نوع الوثيقة: |
thesis |
| اللغة: |
French |
| العلاقة: |
10670/1.061wrd; http://www.theses.fr/2013PA05T008Test |
| الإتاحة: |
http://www.theses.fr/2013PA05T008Test |
| رقم الانضمام: |
edsbas.7B7DB583 |
| قاعدة البيانات: |
BASE |
