التفاصيل البيبلوغرافية
| العنوان: |
Plasmids pSci from Spiroplasma citri GII3 : functional characterization and role in insect transmission ; Les plasmides pSci de Spiroplasma citri GII3 : caractérisation fonctionnelle et rôle dans la transmission par l'insecte vecteur |
| المؤلفون: |
Breton, Marc |
| المساهمون: |
Bordeaux 2, Renaudin, Joël |
| المصدر: |
Theses.fr |
| سنة النشر: |
2009 |
| مصطلحات موضوعية: |
Mollicute phytopathogène, Spiroplasma citri, Plasmides, Réplication, Partition, Incompatibilité, Transmission par insecte, Expression inductible, Plant pathogenic mollicute, Plasmids, Incompatibility, Insect transmission, Inducible expression, envir, stat |
| الوصف: |
Plasmids pSci from Spiroplasma citri GII3 display a mosaic gene organization with highly conserved regions. Through successive deletions, various pSci2 derivatives were constructed and assessed for their ability to replicate. The smallest functional replicon consists of one single CDS (pE) and its flanking, intergenic regions. Furthermore, shuttle (S. citri/E. coli) plasmids, in which the pE gene was disrupted, failed to replicate in S. citri, suggesting that PE is the replication protein. S. citri plasmids were efficiently introduced into S. kunkelii and S. phoeniceum, two plant pathogenic spiroplasmas, the transformation of which had never been described before. Studying stability of various pSci-derived plasmids in the absence of selection pressure strongly suggests the occurrence of an active partition system involving the soj-like gene. Selective incompatibility between a given pSci plasmid and its derivatives was used to remove plasmids from the wild-type strain. As a result, a collection of S. citri GII3 mutants differing in their plasmid contents was produced. Experimental transmission of these mutants through injection to or ingestion by the leafhopper vector will provide new insights into the role of plasmid encoded determinants in the biology of S. citri. First data indicated that pSci6 traG is required for insect transmission of S. citri GII3. In contrast, pSci1-5, encoding adhesin-like proteins, are not essential for both transmission and acquisition. In the frame of this work, new genetic tools have been adapted for use in mollicutes. The tetracycline inducible promoter, Pxyl/tetO2, was used to control spiraline gene in S. citri and M. agalactiae. Also, an in vivo linearization system based on the expression of the I-SceI-endonuclease was used to remove pSci plasmids from S. citri. ; Les plasmides pSci de Spiroplasma citri GII3 possèdent une structure mosaïque avec de nombreuses régions conservées. Des dérivés du plasmide pSci2 ont été produits par délétions successives et leur capacité de ... |
| نوع الوثيقة: |
thesis |
| اللغة: |
French |
| العلاقة: |
10670/1.dxjenr; http://www.theses.fr/2009BOR21673/documentTest |
| الإتاحة: |
http://www.theses.fr/2009BOR21673/documentTest |
| حقوق: |
other |
| رقم الانضمام: |
edsbas.1AAC0EFB |
| قاعدة البيانات: |
BASE |
