Novel puroindoline and grain softness protein alleles in Aegilops species with the C, D, S, M and U genomes
| العنوان: | Novel puroindoline and grain softness protein alleles in Aegilops species with the C, D, S, M and U genomes |
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| المؤلفون: | Guangyuan He, Mark Wilkinson, Peter R. Shewry, Paola Tosi, Mingjie Chen |
| المصدر: | Theoretical and Applied Genetics. 111:1159-1166 |
| بيانات النشر: | Springer Science and Business Media LLC, 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Blotting, Western, Molecular Sequence Data, Locus (genetics), Poaceae, Genome, Complementary DNA, Genetics, Amino Acid Sequence, Gene, Alleles, DNA Primers, Plant Proteins, Southern blot, Base Sequence, biology, Reverse Transcriptase Polymerase Chain Reaction, food and beverages, Sequence Analysis, DNA, General Medicine, biology.organism_classification, Stop codon, Blotting, Southern, genomic DNA, Aegilops, Microscopy, Electron, Scanning, Edible Grain, Sequence Alignment, Agronomy and Crop Science, Biotechnology |
| الوصف: | DNA from six hexaploid, tetraploid and diploid species of Aegilops with the C, D, S, M and U genomes was amplified with specific PCR primers to identify sequences encoding puroindolines (Pins) a and b and grain softness protein (GSP), all of which are encoded by genes at the Ha (hardness) locus, with Ae. tauschii (DD) and bread wheat (T. aestivum) (AABBDD) cv Hiline being studied as controls. Seven new allelic forms of Pin a and Pin b were identified, including forms with mutations within or close to the tryptophan motif. In addition, five new forms of GSP were detected. In all species both genomic DNA from leaves and cDNA from developing grain were analysed. This revealed the presence of both silent genes (with premature stop codons) and multiple genes, with the latter being confirmed by Southern blot analysis. Freeze fracture analysis demonstrated that all except one accession (Ae. sharonensis) were soft textured. However, this difference cannot be accounted for by the sequences of the Pin alleles present in this line. |
| تدمد: | 1432-2242 0040-5752 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b8143f6af8621724df0077688166716aTest https://doi.org/10.1007/s00122-005-0047-7Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....b8143f6af8621724df0077688166716a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14322242 00405752 |
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