High-molecular-weight glutenin subunits in the Cylindropyrum and Vertebrata section of the Aegilops genus and identification of subunits related to those encoded by the Dx alleles of common wheat
| العنوان: | High-molecular-weight glutenin subunits in the Cylindropyrum and Vertebrata section of the Aegilops genus and identification of subunits related to those encoded by the Dx alleles of common wheat |
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| المؤلفون: | Y. Wan, Peter R. Shewry, Kunfan Liu, Dongzhi Wang |
| المصدر: | Theoretical and Applied Genetics. 101:879-884 |
| بيانات النشر: | Springer Science and Business Media LLC, 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | chemistry.chemical_classification, Genetics, biology, Protein subunit, Crassa, food and beverages, General Medicine, biology.organism_classification, Glutenin, Biochemistry, chemistry, Plant protein, Aegilops, biology.protein, Aegilops tauschii, Storage protein, Common wheat, Agronomy and Crop Science, Biotechnology |
| الوصف: | The high-molecular-weight (HMW) glute-nin subunit composition of seven species from the Cylindropyrum and Vertebrata sections of the Aegilops genus was studied using SDS-PAGE and Western blot analysis. Two subunits were detected in Ae. caudata and three in Ae. cylindrica. In both species, subunits showing electrophoretic mobility similar to that of 1Dx2 were present. Western blot analysis using a monoclonal antibody (IFRN 1602) specific for the 1Ax and 1Dx subunits of bread wheat showed that the 1Dx-like subunit of Ae. caudata gave only a weak reaction. This indicates that Ae. caudata expresses subunits which are more distantly related to the 1Dx subunits. Two subunits were detected in each of the 60 accessions of Ae. tauschii, including several 1Dtx subunits showing different electrophoretic mobilities from those of the 1Dx subunits commonly found in bread wheat. All of the 1Dtx subunits reacted strongly with IFRN 1602, confirming their close relationship to the 1Dx subunits of bread wheat. Three subunits were found in Ae. crassa (6 x), four in Ae. ventricosa and Ae. juvenalis and five in Ae. vavilovii. In these four species, the subunits that showed electrophoretic mobility similar, or close, to that of 1Dx2 all reacted with IFRN 1602. In addition, Ae. ventricosa contained a subunit showing electrophoretic mobility slower than that of 1Dx2.2, which also reacted with IFRN 1602. These results suggest that the D-genome component in the multiploid Aegilops species express at least one HMW glutenin subunit that is structurally related to the 1Dx subunits of bread wheat. |
| تدمد: | 1432-2242 0040-5752 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::71ea55ca9560773dab4832cd9d7931ebTest https://doi.org/10.1007/s001220051556Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........71ea55ca9560773dab4832cd9d7931eb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14322242 00405752 |
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