Altered functional properties of tritordeum by transformation with HMW glutenin subunit genes
| العنوان: | Altered functional properties of tritordeum by transformation with HMW glutenin subunit genes |
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| المؤلفون: | Arthur S. Tatham, P. Barcelo, A. Martin, Francisco Barro, S. Steele, L. Rooke, Paul A. Lazzeri, Frank Békés, Peter W. Gras, Roger J. Fido, Peter R. Shewry |
| المصدر: | Theoretical and Applied Genetics. 99:851-858 |
| بيانات النشر: | Springer Science and Business Media LLC, 1999. |
| سنة النشر: | 1999 |
| مصطلحات موضوعية: | chemistry.chemical_classification, biology, Transgene, Protein subunit, food and beverages, Chromosome, General Medicine, biology.organism_classification, Gluten, Transformation (genetics), Glutenin, chemistry, Biochemistry, Botany, Genetics, biology.protein, Storage protein, Hordeum, Agronomy and Crop Science, Biotechnology |
| الوصف: | The high-molecular-weight (HMW) subunits of wheat glutenin are the major determinants of the gluten visco-elasticity that allows wheat doughs to be used to make bread, pasta and other food products. In order to increase the proportions of the HMW subunits, and hence improve breadmaking performance, particle bombardment was used to transform tritordeum, a fertile amphiploid between wild barley and pasta wheat, with genes encoding two HMW glutenin subunits (1Ax1 and 1Dx5). Of the 13 independent transgenic lines recovered (a transformation frequency of 1.4%) six express the novel HMW subunits at levels similar to, or higher than, those of the endogenous subunits encoded on chromosome 1B. Small-scale mixograph analysis of T2 seeds from a line expressing the transgene for 1Dx5 indicated that the addition of novel HMW subunits can result in significant improvements in dough strength and stability, thus demonstrating that transformation can be used to modify the functional properties of tritordeum for improved breadmaking. |
| تدمد: | 1432-2242 0040-5752 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::2c1272a9e4b9f1376ef8eb2a1d242db2Test https://doi.org/10.1007/s001220051305Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........2c1272a9e4b9f1376ef8eb2a1d242db2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14322242 00405752 |
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