TTL Proteins Scaffold Brassinosteroid Signaling Components at the Plasma Membrane to Optimize Signal Transduction in Arabidopsis
| العنوان: | TTL Proteins Scaffold Brassinosteroid Signaling Components at the Plasma Membrane to Optimize Signal Transduction in Arabidopsis |
|---|---|
| المؤلفون: | Naoufal Lakhssassi, David Posé, Araceli G. Castillo, Yansha Li, Cyril Zipfel, Victoriano Valpuesta, Alberto P. Macho, Álvaro García-Moreno, Miguel A. Botella, Jessica Pérez-Sancho, Vitor Amorim-Silva, Jinxing Lin, Alicia Esteban del Valle, Josefa Perez-Rodriguez, Omar Borsani |
| المساهمون: | University of Zurich, Botella, Miguel A |
| المصدر: | Plant Cell |
| بيانات النشر: | Oxford University Press (OUP), 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Scaffold protein, Phosphatase, Arabidopsis, Plant Science, 580 Plants (Botany), Biology, 01 natural sciences, 1307 Cell Biology, 03 medical and health sciences, chemistry.chemical_compound, 10126 Department of Plant and Microbial Biology, Gene Expression Regulation, Plant, Brassinosteroids, 1110 Plant Science, Transcriptional regulation, Brassinosteroid, 10211 Zurich-Basel Plant Science Center, Transcription factor, Research Articles, Arabidopsis Proteins, Cell Membrane, fungi, Membrane Proteins, Cell Biology, biology.organism_classification, Cell biology, Tetratricopeptide, 030104 developmental biology, chemistry, Signal transduction, Signal Transduction, 010606 plant biology & botany |
| الوصف: | Brassinosteroids (BRs) form a group of steroidal hormones essential for plant growth, development, and stress responses. BRs are perceived extracellularly by plasma membrane receptor-like kinases that activate an interconnected signal transduction cascade, leading to the transcriptional regulation of BR-responsive genes. TETRATRICOPEPTIDE THIOREDOXIN-LIKE (TTL) genes are specific for land plants, and their encoded proteins are defined by the presence of protein–protein interaction motives, that is, an intrinsic disordered region at the N terminus, six tetratricopeptide repeat domains, and a C terminus with homology to thioredoxins. TTL proteins thus likely mediate the assembly of multiprotein complexes. Phenotypic, molecular, and genetic analyses show that TTL proteins are positive regulators of BR signaling in Arabidopsis (Arabidopsis thaliana). TTL3 directly interacts with a constitutively active BRASSINOSTEROID INSENSITIVE1 (BRI1) receptor kinase, BRI1-SUPPRESSOR1 phosphatase, and the BRASSINAZOLE RESISTANT1 transcription factor and associates with BR-SIGNALING KINASE1, BRASSINOSTEROID INSENSITIVE2 kinases, but not with BRI1-ASSOCIATED KINASE1. A functional TTL3-green fluorescent protein (GFP) shows dual cytoplasmic plasma membrane localization. Depleting the endogenous BR content reduces plasma membrane localization of TTL3-GFP, while increasing BR content causes its plasma membrane relocalization, where it strengthens the association of BR signaling components. Our results reveal that TTL proteins promote BR responses and suggest that TTL proteins may function as scaffold proteins by bringing together cytoplasmic and plasma membrane BR signaling components. |
| وصف الملف: | tpc.19.00150.full.pdf - application/pdf; application/pdf |
| تدمد: | 1532-298X 1040-4651 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f940cb15dc275f0a63fe17ea1375f45cTest https://doi.org/10.1105/tpc.19.00150Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f940cb15dc275f0a63fe17ea1375f45c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1532298X 10404651 |
|---|