NanoSIMS analysis of arsenic and selenium in cereal grain
| العنوان: | NanoSIMS analysis of arsenic and selenium in cereal grain |
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| المؤلفون: | Enzo Lombi, Malcolm J. Hawkesford, Peter R. Shewry, Markus Schröder, Chris R. M. Grovenor, Katie L. Moore, Fang-Jie Zhao, Steve P. McGrath |
| المساهمون: | Moore, Katie L, Schroder, Markus, Lombi, Enzo, Zhao, F, McGrath, S, Hawkesford, Malcolm J, Shewry, Peter R, Grovenor, Chris RM |
| المصدر: | The New phytologist. 185(2) |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Physiology, Starch, Spectrometry, Mass, Secondary Ion, chemistry.chemical_element, Plant Science, Arsenic, Endosperm, Selenium, chemistry.chemical_compound, Aleurone, Botany, Food science, Triticum, Oryza sativa, Chemistry, Plant Sciences, Trace element, Proteins, Spectrometry, X-Ray Emission, food and beverages, Oryza, Secondary ion mass spectrometry, Seeds |
| الوصف: | Summary •Cereals are an important source of selenium (Se) to humans and many people have inadequate intakes of this essential trace element. Conversely, arsenic (As) is toxic and may accumulate in rice grain at levels that pose a health risk. Knowledge of the localization of selenium and arsenic within the cereal grain will aid understanding of their deposition patterns and the impact of processes such as milling. •High-resolution secondary ion mass spectrometry (NanoSIMS) was used to determine the localization of Se in wheat (Triticum aestivum) and As in rice (Oryza sativa). Combined synchrotron X-ray fluorescence (S-XRF) and NanoSIMS analysis utilized the strengths of both techniques. •Selenium was concentrated in the protein surrounding the starch granules in the starchy endosperm cells and more homogeneously distributed in the aleurone cells but with Se-rich hotspots. Arsenic was concentrated in the subaleurone endosperm cells in association with the protein matrix rather than in the aleurone cells. NanoSIMS indicated that the high intensity of As identified in the S-XRF image was localized in micron-sized hotspots near the ovular vascular trace and nucellar projection. •This is the first study showing subcellular localization in grain samples containing parts per million concentrations of Se and As. There is good quantitative agreement between NanoSIMS and S-XRF. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1469-8137 0028-646X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::446b62b09c1cb7f03b3d74288895f564Test http://ora.ox.ac.uk/objects/uuid:4c4d795a-761a-4b00-88bf-9b154339e8c0Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....446b62b09c1cb7f03b3d74288895f564 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14698137 0028646X |
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