Deterioration of endothelial and smooth muscle cell function in DCD kidneys after static cold storage in IGL-1 or UW
| العنوان: | Deterioration of endothelial and smooth muscle cell function in DCD kidneys after static cold storage in IGL-1 or UW |
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| المؤلفون: | Mark-Hugo J. Maathuis, Rutger J. Ploeg, Martijn de Groot, Henri G. D. Leuvenink |
| المساهمون: | Groningen Institute for Organ Transplantation (GIOT) |
| المصدر: | Journal of Surgical Research, 152(2), 231-237. ACADEMIC PRESS INC ELSEVIER SCIENCE |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Male, Adenosine, donation after cardiac death, Kidney, Muscle, Smooth, Vascular, DEPENDENT RELAXATION, isolated perfused kidney, HYPOTHERMIC MACHINE PERFUSION, Insulin, OF-WISCONSIN SOLUTION, REPERFUSION INJURY, warm ischemia, RABBIT AORTA, Vasomotor, Anatomy, Glutathione, Tissue Donors, ISCHEMIA, Cold Temperature, medicine.anatomical_structure, Tissue and Organ Harvesting, Cardiology, cardiovascular system, medicine.symptom, medicine.medical_specialty, Allopurinol, preservation, Organ Preservation Solutions, Ischemia, kidney transplantation, Cold storage, vasomotor function, Renal Circulation, Constriction, PRESERVATION SOLUTION, Raffinose, POLYETHYLENE-GLYCOL, Internal medicine, Cadaver, medicine, Animals, Viaspan, ORGAN-PRESERVATION, business.industry, medicine.disease, Rats, Inbred F344, Rats, RAT-KIDNEY, Vasoconstriction, Reperfusion, Surgery, Endothelium, Vascular, business, Reperfusion injury |
| الوصف: | Background. Kidneys obtained from donors after cardiac death are damaged by the combination of warm and cold ischemia. Although the parenchymal damage of these kidneys is well studied, little is known about the functional effects of warm and cold ischemia on the renal vascular bed. We compared kidney preservation using the new extracellular-type cold storage solution from Institut Georges Lopez (IGL-1) with the University of Wisconsin solution (UW) and focused on vasomotor functions.Methods. The influence of warm and cold ischemia on vasomotor functions was studied in an isolated perfused kidney model. Six groups of donation after cardiac death donor kidneys were studied with warm ischemia of 0, 15, and 30 min followed by 0 or 24 h cold storage preservation in IGL-1 or UW at 4 degrees C. Endothelial dependent vasodilation was studied using acetylcholine, smooth muscle cell (SMC) constriction was assessed using phenylephrine, and finally endothelial independent relaxation was tested using papaverine-sulfate.Results. SMCs were significantly affected by cold ischemia showing a 50%. reduction of phenylephrine mediated constriction after preservation. Additional warm ischemia did not affect SMCs. After UW preservation endothelial dependent vasodilation was only significantly reduced when the combination of warm and cold ischemia was present. IGL-1 preserved kidneys showed a reduction in endothelial dependent vasodilation after isolated warm ischemia. Both preservation solutions rendered equal results after 24 h preservation.Conclusion. Vasomotor functions are negatively influenced by the combination of warm and cold ischemia. Both IGL-1 and UW performed equally in preserving vasomotor functions. The interesting finding of the rapid decline of SMC function might point at the first step toward intimal hyperplasia as seen in late transplant dysfunction. (C) 2009 Elsevier Inc. All rights reserved. |
| اللغة: | English |
| تدمد: | 1095-8673 0022-4804 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6666013cdefdffbf9eda9d55b16a16d5Test http://ora.ox.ac.uk/objects/uuid:9de08f49-7a3b-4a27-832a-8d467bfc9333Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6666013cdefdffbf9eda9d55b16a16d5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10958673 00224804 |
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