Enriched uranium affects the expression of vitamin D receptor and retinoid X receptor in rat kidney
| العنوان: | Enriched uranium affects the expression of vitamin D receptor and retinoid X receptor in rat kidney |
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| المؤلفون: | Jocelyne Aigueperse, Jean-Marc A. Lobaccaro, Patrick Gourmelon, Maâmar Souidi, Line Grandcolas, Yann Gueguen, E. Tissandie |
| المساهمون: | Service de RadioBiologie et d'Epidémiologie (IRSN/DRPH/SRBE), Institut de Radioprotection et de Sûreté Nucléaire (IRSN), Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Radiobiologie et épidémiologie (DRPH/SRBE) |
| المصدر: | Journal of Steroid Biochemistry and Molecular Biology Journal of Steroid Biochemistry and Molecular Biology, 2008, 110 (3-5), pp.263-268. ⟨10.1016/j.jsbmb.2007.11.002⟩ |
| بيانات النشر: | Elsevier BV, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Male, polymerase chain reaction, [SDV]Life Sciences [q-bio], Endocrinology, Diabetes and Metabolism, Messenger, Clinical Biochemistry, Drug Evaluation, Preclinical, 010501 environmental sciences, Kidney, 01 natural sciences, Biochemistry, Calcitriol receptor, Western blotting, Rats, Sprague-Dawley, Endocrinology, calcium transport, homeostasis, Receptors, calcium blood level, rat, Vitamin D, 0303 health sciences, education.field_of_study, vitamin blood level, messenger RNA, vanilloid receptor 5, article, Brain, water contamination, Preclinical, retinoid X receptor alpha, enzyme activity, medicine.anatomical_structure, kidney tubule absorption, cell nucleus receptor, Uranium, Molecular Medicine, Drug, medicine.medical_specialty, colecalciferol, Duodenum, animal experiment, Population, biological activity, gene sequence, Biology, Retinoid X receptor, animal tissue, long term exposure, in vivo study, Dose-Response Relationship, 03 medical and health sciences, vitamin D metabolism, Calcitriol, calbindin, Internal medicine, medicine, Vitamin D and neurology, vitamin D receptor, Animals, controlled study, RNA, Messenger, education, protein expression, Molecular Biology, 030304 developmental biology, 0105 earth and related environmental sciences, calcium, nonhuman, Dose-Response Relationship, Drug, Retinoid X receptor alpha, Rattus, nucleotide sequence, Cell Biology, intestine absorption, Rats, Retinoid X Receptors, Gene Expression Regulation, Nuclear receptor, protein blood level, Receptors, Calcitriol, Drug Evaluation, RNA, Sprague-Dawley, Hormone |
| الوصف: | An increasing awareness of the radiological impact of the nuclear power industry and other nuclear technologies is observed nowadays on general population. This led to renew interest to assess the health impact of the use of enriched uranium (EU). The aim of this work was to investigate in vivo the effects of a chronic exposure to EU on vitamin D3 metabolism, a hormone essential in mineral and bone homeostasis. Rats were exposed to EU in their drinking water for 9 months at a concentration of 40 mg l-1 (1 mg/rat day). The contamination did not change vitamin D plasma level. Vitamin D receptor (vdr) and retinoid X receptor alpha (rxrα), encoding nuclear receptors involved in the biological activities of vitamin D, showed a lower expression in kidney, while their protein levels were paradoxically increased. Gene expression of vitamin D target genes, epithelial Ca2+ channel 1 (ecac1) and Calbindin-D28k (cabp-d28k), involved in renal calcium transport were decreased. Among the vitamin D target organs examined, these molecular modifications occurred exclusively in the kidney, which confirms that this organ is highly sensitive to uranium exposure. In conclusion, this study showed that a chronic exposure to EU affects both mRNA and protein expressions of renal nuclear receptors involved in vitamin D metabolism, without any modification of the circulating vitamin D. © 2008 Elsevier Ltd. All rights reserved. |
| تدمد: | 0960-0760 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::204cca7d78e069693149e3820688b623Test https://doi.org/10.1016/j.jsbmb.2007.11.002Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....204cca7d78e069693149e3820688b623 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09600760 |
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