المؤلفون: Yh, Yang, Hutchinson P, Leech M, Eric Morand

المصدر: Europe PubMed Central

مصطلحات موضوعية: Male, Rats, Sprague-Dawley, Leukocytes, Animals, Edema, Adrenalectomy, Extremities, Flow Cytometry, Arthritis, Experimental, Lymphocyte Subsets, Annexin A1, Rats

الوصف: Lipocortin 1 is a mediator of the antiinflammatory actions of therapeutic glucocorticoids. ENdogenous glucocorticoids modulate inflammatory arthritides including rheumatoid and adjuvant arthritis (AA), but the role of lipocortin 1 in this phenomenon is not known. We studied the effects of endogenous glucocorticoids on adjuvant arthritis and leukocyte lipocortin 1 content.Adrenalectomy of sham adrenalectomy was performed 2 days before adjuvant injection in 170 g inbred Sprague-Dawley rats. Peripheral blood was obtained and disease severity assessed by delta paw volume and clinical score 14 days later. Leukocyte subset lipocortin 1 content was determined by double labeling permeabilization flow cytometry using specific monoclonal antibodies.Lipocortin 1 fluorescence was readily detected in control rat peripheral blood cells labeled with OX-1 (pan-leukocyte), OX-19 (CD5), W3/25 (CD4), and OX-8 (CD8). Lipocortin 1 fluorescence was significantly greater in polymorphonuclear leukocytes (PMN) (RP3; p0.01). Induction of AA was accompanied by significant increases in lipocortin 1 (p0.001) in all subsets. Sham adrenalectomy induced no significant change in AA rat leukocyte lipocortin 1. Adrenalectomy induced significant exacerbation of AA disease severity compared to sham operation (delta paw volume 1.43 +/- 0.1 vs 1.13 +/- 0 ml; p0.05). Adrenalectomy was also associated with significant reduction in lipocortin 1 content in all leukocyte subsets except PMN. Leukocyte lipocortin 1 content exhibited significant negative correlation with clinical disease severity (R2 = -0.55; p0.05).Endogenous glucocorticoids modulate leukocyte expression of lipocortin 1 in inflammatory disease, and reduced lipocortin 1 may be involved in the exacerbation of AA by adrenalectomy.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::1c06da3531d23b526ae5393656e70f07Test
https://pubmed.ncbi.nlm.nih.gov/9292800Test

المؤلفون: Rs, Axtens, Mh, Miller, Go, Littlejohn, Dj, Topliss, Eric Morand

المصدر: Europe PubMed Central

مصطلحات موضوعية: Adult, Aged, 80 and over, Male, Middle Aged, Prognosis, Recurrence, Risk Factors, Antibodies, Anticardiolipin, Humans, Lupus Erythematosus, Systemic, Female, Vascular Diseases, Fetal Death, Aged

الوصف: To examine the usefulness of a single measurement of anticardiolipin antibodies (aCL) in systemic lupus erythematosus (SLE) in routine clinical practice.All 127 patients with SLE currently followed by our rheumatology unit had an aCL measurement on routine clinic review. Their charts were then reviewed for specific disease manifestations. Basic statistical correlations of the aCL result and the specific disease manifestations were performed, and the clinical utility of the aCL test assessed using Bayesian analysis.aCL was positive (2 SD) in 24% and was associated with recurrent fetal loss, thrombosis, cerebrovascular disease, livedo reticularis and digital infarcts. Bayesian analysis showed that a single positive aCL test increased the relative and absolute risk of all the above complications. The criterion of aCL positivity as15 units (2 SD) was associated with the highest relative risk.A single positive aCL test in routine management of SLE is a useful predictor of important clinical events.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::8d9c823e93c3ccd7a998e97dcf8e3afcTest
https://pubmed.ncbi.nlm.nih.gov/8151597Test