Growth Factor Activity of Endothelin-1 in Primary Astrocytes Mediated by Adhesion-Dependent and -Independent Pathways
| العنوان: | Growth Factor Activity of Endothelin-1 in Primary Astrocytes Mediated by Adhesion-Dependent and -Independent Pathways |
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| المؤلفون: | Ignacio A. Romero, Nathalie Chaverot, A. Donny Strosberg, Peter Adamson, Sylvie Cazaubon, Pierre-Olivier Couraud, Jean-Antoine Girault |
| المصدر: | The Journal of Neuroscience. 17:6203-6212 |
| بيانات النشر: | Society for Neuroscience, 1997. |
| سنة النشر: | 1997 |
| مصطلحات موضوعية: | rho GTP-Binding Proteins, MAPK/ERK pathway, Fluorides, chemistry.chemical_compound, Phosphorylation, Aluminum Compounds, Cells, Cultured, Cytoskeleton, Protein Kinase C, Cerebral Cortex, Endothelin-1, biology, General Neuroscience, Articles, Protein-Tyrosine Kinases, Cell biology, ErbB Receptors, src-Family Kinases, Rabbits, Mitogen-Activated Protein Kinases, Cell Division, Protein Binding, Signal Transduction, Proto-oncogene tyrosine-protein kinase Src, PTK2, Nerve Tissue Proteins, src Homology Domains, Focal adhesion, Fetus, GTP-Binding Proteins, Animals, Paxillin, Adaptor Proteins, Signal Transducing, GRB2 Adaptor Protein, Proteins, Tyrosine phosphorylation, DNA, Focal Adhesion Kinase 2, Phosphoproteins, Actin cytoskeleton, Actins, Corpus Striatum, Rats, Cytoskeletal Proteins, chemistry, Astrocytes, Focal Adhesion Kinase 1, Focal Adhesion Protein-Tyrosine Kinases, biology.protein, Tyrosine, Stress, Mechanical, Cell Adhesion Molecules |
| الوصف: | Endothelin-1 (ET-1) has been shown to induce DNA synthesis in primary astrocytes by stimulating the extracellular signal-regulated kinase (ERK) pathway. To clarify the mechanisms responsible for the anchorage-dependent growth of astrocytes, the relationships between cell adhesion and ERK activation were investigated. Here it is reported that ET-1 promotes the formation of stress fibers and focal adhesions and the tyrosine phosphorylation of focal adhesion kinase (FAK) and paxillin, as well as Src activation and association of phosphorylated FAK with Grb2. Pretreatment of astrocytes with cytochalasin D or C3-transferase, which inhibits actin polymerization or Rho activity, respectively, prevented the activation/phosphorylation of Src, FAK, and paxillin after ET-1 stimulation; by contrast, the ERK pathway was not significantly affected. This differential activation of FAK/Src and ERK pathways was also observed with astrocytes 10 and 60 min after replating on poly-L-ornithine-precoated dishes. Collectively, these findings indicate that activation of FAK and Src is dependent on actin cytoskeleton integrity, Rho activation, and adhesion to extracellular matrix, whereas ERK activation is independent of these intracellular events and seems to correlate with activation of the newly identified protein tyrosine kinase PYK2. Induction of DNA synthesis by ET-1, however, was reduced dramatically in astrocytes pretreated with either cytochalasin D or C3-transferase. This study provides a demonstration of Rho- and adhesion-dependent activation of FAK/Src, which collaborates with adhesion-independent activation of PYK2/ERK for DNA synthesis in ET-1-stimulated astrocytes. |
| تدمد: | 1529-2401 0270-6474 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::10e2a60a577303540bba06d99e35b4e5Test https://doi.org/10.1523/jneurosci.17-16-06203.1997Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....10e2a60a577303540bba06d99e35b4e5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15292401 02706474 |
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