E2A Expression Stimulates Ig Hypermutation
| العنوان: | E2A Expression Stimulates Ig Hypermutation |
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| المؤلفون: | Ulrike Schoetz, Manuela Cervelli, Yan-Dong Wang, Jean-Marie Buerstedde, Petra Fiedler |
| المساهمون: | Schoetz, U, Cervelli, Manuela, Wang, Yd, Fiedler, P, Buerstedde, Jm |
| المصدر: | The Journal of Immunology. 177:395-400 |
| بيانات النشر: | The American Association of Immunologists, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Genetic Markers, DNA, Complementary, activation-induced cytidine deaminase, Molecular Sequence Data, Immunology, Ig hypermutation, Receptors, Antigen, B-Cell, Somatic hypermutation, DT 40 cell, Cell Line, Avian Proteins, chemistry.chemical_compound, E2A gene, Transcription (biology), Cytidine Deaminase, Activation-induced (cytidine) deaminase, Animals, Immunology and Allergy, Gene silencing, Gene Silencing, Enhancer, Gene, E12 protein, Base Sequence, biology, Genetic Complementation Test, Cytidine, Sequence Analysis, DNA, Cytidine deaminase, Molecular biology, Clone Cells, E47 protein, chemistry, biology.protein, Immunoglobulin Light Chains, Somatic Hypermutation, Immunoglobulin, TCF Transcription Factors, Chickens |
| الوصف: | Ig hypermutation is limited to a region of ∼2 kb downstream of the transcription start sites of the Ig loci. The process requires transcription and the presence of Ig enhancer sequences, and is initiated by the activation-induced cytidine deaminase (AID)-mediated deamination of cytidine bases. It remains unknown why AID causes mutations selectively in the Ig genes and not in most other transcribed loci of B cells. In this study, we report that the inactivation of the E2A gene strongly reduces the rate of Ig L chain mutations in the chicken B cell line DT40 without affecting the levels of surface Ig or AID expression. The defect is complemented by the expression of cDNAs corresponding to either of the two E2A splice variants E12 or E47. The results suggest that E2A-encoded proteins enhance Ig hypermutation by recruitment of AID to the Ig loci. |
| تدمد: | 1550-6606 0022-1767 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::372c52f92fdfa6ef710faac4ad3162bbTest https://doi.org/10.4049/jimmunol.177.1.395Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....372c52f92fdfa6ef710faac4ad3162bb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15506606 00221767 |
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