Comparison of DNA sensing by non-immune cells and tissues
| العنوان: | Comparison of DNA sensing by non-immune cells and tissues |
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| المؤلفون: | Loree Heller, Katarina Znida, Masa Bosnjak, Amanda Sales Conniff, Tanja Jesenko, Bostjan Markelc, Jared Tur, Nina Semenova, Maja Cemazar |
| المصدر: | The Journal of Immunology. 208:164.06-164.06 |
| بيانات النشر: | The American Association of Immunologists, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Immunology, Immunology and Allergy |
| الوصف: | Pattern recognition receptors (PRRs) are nearly ubiquitous in both immune and non-immune mammalian cells. PRRs detect pathogen invasion as well as internal damage, inducing the secretion of pro-inflammatory molecules and cell death. Members of the PRR subset that detect intracellular DNA are termed DNA sensors. We examined DNA sensing in non-immune cells and their equivalent tissues using electroporation. This physical delivery method mitigates the need for viral or chemical agents that could confound the source of PRR activation. We used RT-qPCR and/or RNA-Seq to quantify mRNAs encoding DNA sensors and their signaling pathways and western blots, ELISAs and bead arrays to quantify related proteins. Several tumor cell types and non-tumor cells, including keratinocytes, fibroblasts, and myoblasts, expressed different DNA sensors at baseline and responded to backbone plasmid DNA delivery with individual DNA sensor signatures. Some similarities were observed among all these cell types, including upregulation of the DNA sensors ZBP1, DDX60, and Ifi204, the mouse analog of human Ifi16. Each cell type secreted a range of pro-inflammatory molecules including Type I interferon. DNA electroporation to B16-F10 melanomas, skin, and skeletal muscle induced unique cytokine and chemokine signatures, paralleling cellular signatures. ZBP1, DDX60, and Ifi204 were upregulated in skin and muscle; however, no significant regulation was observed in tumors. In muscle particularly, this may be due to the homogeneity of the tissue; tumors are inherently heterogeneous, producing an assortment of individual cellular responses. Supported by NIH grant R01CA196796. |
| تدمد: | 1550-6606 0022-1767 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::f8ba6c4b8773243f364a5d557bfddcaaTest https://doi.org/10.4049/jimmunol.208.supp.164.06Test |
| رقم الانضمام: | edsair.doi...........f8ba6c4b8773243f364a5d557bfddcaa |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15506606 00221767 |
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