Molecular Characterization of the D Surface Protein Gene Subfamily in Paramecium primaurelia
| العنوان: | Molecular Characterization of the D Surface Protein Gene Subfamily in Paramecium primaurelia |
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| المؤلفون: | Francois Caron, Florence M. Bourgain-Guglielmetti |
| المصدر: | The Journal of Eukaryotic Microbiology. 43:303-313 |
| بيانات النشر: | Wiley, 1996. |
| سنة النشر: | 1996 |
| مصطلحات موضوعية: | Gene Rearrangement, Paramecium, Base Sequence, Pseudogene, Molecular Sequence Data, Nucleic acid sequence, Gene Expression, Antigens, Protozoan, Gene rearrangement, Biology, Microbiology, Molecular biology, Open reading frame, Oligodeoxyribonucleotides, Complementary DNA, Antigens, Surface, Gene expression, Animals, Coding region, Amino Acid Sequence, Cloning, Molecular, DNA Probes, Gene |
| الوصف: | When paramecium primaurelia expresses the D serotype, a major high molecular weight mRNA species is detected in the cytoplasm. Using the cDNA derived from this mRNA as a probe, three very similar genes, D alpha, D beta and D gamma, were cloned. Of these three genes, we show that only the D alpha mRNA is present in the cytoplasm of cells expressing the D serotype and corresponds to the major mRNA species. The nucleotide sequence of the entire coding region of the D alpha gene, as well as the upstream and downstream sequences, has been determined. The 7632-nucleotide open reading frame encodes a putative protein that displays the characteristic cysteine residue periodicity of Paramecium surface antigens but does not contain central tandemly repeated sequences. Partial sequences of the two nonexpressed genes D beta and D gamma indicate a high percentage of identity (90%-95%) with the D alpha gene, suggesting that D beta and D gamma genes are either very similar surface protein genes whose transcription is repressed trough mutual exclusion, or perhaps are pseudogenes. A region of variable DNA rearrangement was identified 1 kb upstream of the D gamma gene. This macronuclear region arises from the same micronuclear locus by alternative excision of internal eliminated sequences during macronuclear development. |
| تدمد: | 1550-7408 1066-5234 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a0b84a07ad22fb3c05a9c1fc5451ca18Test https://doi.org/10.1111/j.1550-7408.1996.tb03993.xTest |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....a0b84a07ad22fb3c05a9c1fc5451ca18 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15507408 10665234 |
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