دورية
Aquaporins and Fetal Membranes From Diabetic Parturient Women: Expression Abnormalities and Regulation by Insulin
| العنوان: | Aquaporins and Fetal Membranes From Diabetic Parturient Women: Expression Abnormalities and Regulation by Insulin |
|---|---|
| المؤلفون: | Bouvier, Damien, Rouzaire, Marion, Marceau, Geoffroy, Prat, Cécile, Pereira, Bruno, Lemarié, Romain, Deruelle, Philippe, Fajardy, Isabelle, Gallot, Denis, Blanchon, Loïc, Vambergue, Anne, Sapin, Vincent |
| المصدر: | The Journal of Clinical Endocrinology & Metabolism; October 2015, Vol. 100 Issue: 10 pE1270-E1279, 10p |
| مستخلص: | Context:During pregnancy, aquaporins (AQPs) expressed in fetal membranes are essential for controlling the homeostasis of the amniotic volume, but their regulation by insulin was never explored in diabetic women.Objective:The aim of our study was to investigate the involvement of AQPs 1, 3, 8, and 9 expressed in fetal membranes in diabetic parturient women and the control of their expression by insulin.Design and Participants:From 129 fetal membranes in four populations (controls, type 1, type 2 [T2D], and gestational diabetes [GD]), we established an expression AQP profile. In a second step, the amnion was used to study the control of the expression and functions of AQPs 3 and 9 by insulin.Main Outcomes and Measures:The expression of transcripts and proteins of AQPs was studied by quantitative RT-PCR and ELISA. We analyzed the regulation by insulin of the expression of AQPs 3 and 9 in the amnion. A tritiated glycerol test enabled us to measure the impact of insulin on the functional characteristics. Using an inhibitor of phosphatidylinositol 3-kinase, we analyzed the insulin intracellular signaling pathway.Results:The expression of AQP3 protein was significantly weaker in groups T2D and GD. In nondiabetic fetal membranes, we showed for the amnion (but not for the chorion) a significant repression by insulin of the transcriptional expression of AQPs 3 and 9, which was blocked by a phosphatidylinositol 3-kinase inhibitor.Conclusion:In fetal membranes, the repression of AQP3 protein expression and functions observed in vivo is allowed by the hyperinsulinism described in pregnant women with T2D or GD. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 0021972X 19457197 |
|---|---|
| DOI: | 10.1210/jc.2015-2057 |