The transcription activator steroidogenic factor-1 is preferentially expressed in the human pituitary gonadotroph
| العنوان: | The transcription activator steroidogenic factor-1 is preferentially expressed in the human pituitary gonadotroph |
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| المؤلفون: | Keith L. Parker, Bruce Cao, Margaret Wong, Ana-Maria Bamberger, Shereen Ezzat, Sylvia L. Asa |
| المصدر: | The Journal of Clinical Endocrinology & Metabolism. 81:2165-2170 |
| بيانات النشر: | The Endocrine Society, 1996. |
| سنة النشر: | 1996 |
| مصطلحات موضوعية: | Adenoma, Adult, Male, Steroidogenic factor 1, endocrine system, Pituitary gland, medicine.medical_specialty, Adolescent, Transcription, Genetic, endocrine system diseases, Somatotropic cell, medicine.drug_class, Endocrinology, Diabetes and Metabolism, Clinical Biochemistry, Fushi Tarazu Transcription Factors, Receptors, Cytoplasmic and Nuclear, Biology, Steroidogenic Factor 1, Polymerase Chain Reaction, Biochemistry, Prolactin cell, Endocrinology, Pituitary Gland, Anterior, Pituitary adenoma, Internal medicine, medicine, Humans, Pituitary Neoplasms, Tissue Distribution, Cells, Cultured, Aged, Homeodomain Proteins, Biochemistry (medical), Middle Aged, medicine.disease, Immunohistochemistry, DNA-Binding Proteins, stomatognathic diseases, medicine.anatomical_structure, Female, Corticotropic cell, Gonadotropin, Gonadotropins, Transcription Factors |
| الوصف: | Steroidogenic factor-1 (SF-1), also known as adrenal-4-binding protein, is a transcription factor that is important for the differentiation of steroidogenic tissues. We investigated whether SF-1 is expressed in specific hormone-producing cell types in the human pituitary and its adenomas. Pituitary adenomas (n = 35) were collected at the time of surgery, and normal adenohypophyses were obtained from autopsies; 3 corticotroph adenomas were excluded because pit-1 messenger ribonucleic acid (mRNA) expression indicated contamination by nontumorous elements. Expression of SF-1 mRNA was determined by reverse transcription-PCR. SF-1 protein was localized with immunocytochemistry. By reverse transcription-PCR, SF-1 mRNA was found in the nontumorous pituitary and in pituitary adenomas expressing gonadotropins. All 8 gonadotroph adenomas had a strong signal for SF-1. SF-1 mRNA was also detected in 2 of 3 corticotroph adenomas, 2 of 13 somatotroph/mammosomatotroph adenomas, 1 of 6 lactotroph adenomas, and 2 silent subtype 3 adenomas; however, in most of the positive tumors there was also positivity for FSH beta and/or LH beta mRNA, suggesting that contaminating nontumorous gonadotrophs may be the source of SF-1 mRNA signal. SF-1 protein was localized by immunocytochemistry in the nuclei of scattered cells of the nontumorous adenohypophysis that were shown to be gonadotrophs with double immunostaining and in gonadotroph adenomas; nuclear staining was not found in other adenoma types, except in areas shown to contain trapped nontumorous tissue. We conclude that SF-1 expression correlates with the expression of gonadotropins. These findings implicate SF-1 as a cell-specific transcription factor that may regulate gonadotroph differentiation in the pituitary. |
| تدمد: | 1945-7197 0021-972X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::63324490bad2920fcfb2bf1f280e9588Test https://doi.org/10.1210/jcem.81.6.8964846Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....63324490bad2920fcfb2bf1f280e9588 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19457197 0021972X |
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