Expression and Function of the Costimulatory Receptor SLAMF1 Is Altered in Lymphocytes From Patients With Autoimmune Thyroiditis
| العنوان: | Expression and Function of the Costimulatory Receptor SLAMF1 Is Altered in Lymphocytes From Patients With Autoimmune Thyroiditis |
|---|---|
| المؤلفون: | Roberto González-Amaro, Ana Serrano-Somavilla, Miguel Sampedro-Núñez, Ana M. Ramos-Leví, Marlen Vitales-Noyola, Carmelina Di Pasquale, Mónica Marazuela, Rebeca Martínez-Hernández |
| المصدر: | The Journal of Clinical Endocrinology & Metabolism. 102:672-680 |
| بيانات النشر: | The Endocrine Society, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Adult, Male, 0301 basic medicine, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, Graves' disease, Antigens, CD19, Clinical Biochemistry, Thyroid Gland, Hashimoto Disease, T-Lymphocytes, Regulatory, Biochemistry, Peripheral blood mononuclear cell, Thyroiditis, CD19, Autoimmune thyroiditis, 03 medical and health sciences, 0302 clinical medicine, Endocrinology, Signaling Lymphocytic Activation Molecule Family Member 1, Internal medicine, Humans, Medicine, Prospective Studies, IL-2 receptor, B-Lymphocytes, biology, business.industry, Biochemistry (medical), Thyroid, Middle Aged, medicine.disease, Graves Disease, Cross-Sectional Studies, 030104 developmental biology, medicine.anatomical_structure, Immunology, biology.protein, Th17 Cells, Female, business, 030215 immunology |
| الوصف: | Signaling lymphocytic activation molecule family 1 (SLAMF1) is a costimulatory receptor expressed by most immune cells. Its role in autoimmune thyroid disease (AITD) is not well known.To analyze the expression and function of the costimulatory receptor SLAMF1 in lymphocytes of patients with AITD.Cross-sectional, prospective, single-center study.Department of Endocrinology, Hospital Universitario de la Princesa, Madrid.Twenty-eight patients with AITD (17 with Graves disease and 11 with Hashimoto thyroiditis) and 21 controls.Multiparametric flow cytometry and immunofluorescence techniques to analyze the expression of SLAMF1 in peripheral blood (n = 28) and thyroid tissue (n = 5) mononuclear cells. Assay of inhibition of cellular proliferation to study the function of SLAMF1 in CD4+CD25+ T regulatory (Treg) cells.Expression levels and the function of SLAMF1 in lymphocytes in AITD patients and controls.Expression of SLAMF1 was significantly increased in peripheral blood CD4+, T helper 17, and CD19+ B cells from AITD patients. Immunofluorescence microscopy detected the presence of SLAMF1+ lymphocytes in thyroid inflammatory cell infiltrate. Functional studies showed that SLAMF1 engagement in Treg cells increased their suppressive function in healthy controls but not in AITD patients.The altered expression of SLAMF1, as well as its defective function observed in patients with AITD, may have a relevant role in the defective immune-regulatory function observed in this condition. |
| تدمد: | 1945-7197 0021-972X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::67d7b59a4b8e3f8fe5fdd38b11fc8870Test https://doi.org/10.1210/jc.2016-2322Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....67d7b59a4b8e3f8fe5fdd38b11fc8870 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19457197 0021972X |
|---|