Molecular Cloning and Nucleotide Sequence of the Gramicidin S Synthetase 1 Gene1
| العنوان: | Molecular Cloning and Nucleotide Sequence of the Gramicidin S Synthetase 1 Gene1 |
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| المؤلفون: | Toshitaka Minetoki, Masayuki Kanda, Toshitsugu Kurotsu, Kazuko Hori, Yoshitaka Saito, Jun-ichi Furuyama, Setsuko Miura, Kaori Okamura, Yoshihiro Yamamoto |
| المصدر: | The Journal of Biochemistry. 106:639-645 |
| بيانات النشر: | Oxford University Press (OUP), 1989. |
| سنة النشر: | 1989 |
| مصطلحات موضوعية: | chemistry.chemical_classification, Nucleic acid sequence, General Medicine, Molecular cloning, Biology, Biochemistry, Molecular biology, Amino acid, Gene product, chemistry.chemical_compound, chemistry, Tyrocidine, Gramicidin, Molecular Biology, Gene, Peptide sequence |
| الوصف: | The entire gene for gramicidin S synthetase 1 (GS 1) was cloned into the plasmid vector pUC18, and the nucleotide sequences of the GS 1 gene and its flanking region were determined. The full-length clone was 4,539 base pairs long and had an open reading frame of 3,294 nucleotides coding for 1,098 amino acids. The calculated molecular weight of 123,474 agreed with the apparent molecular weight of 120,000 found in SDS-PAGE of GS 1 from B. brevis. The nucleotide sequence of GS 1 gene was highly homologous to that of tyrocidine synthetase 1. The overall similarity between the deduced amino acid sequences of the two genes was 57.5%. The gene product of clone GS309 was easily purified to an essentially homogeneous state by ammonium sulfate fractionation followed by DEAE-Sepharose CL-6B, Ultrogel AcA-34, and second DEAE-Sepharose CL-6B column chromatography. The purified protein catalyzed the D-phenylalanine-dependent ATP-32PPi exchange reaction which is specific for GS 1 activity, and the specific activity of the purified product was nearly the same as the purified GS 1 from B. brevis. The product also showed a weak phenylalanine racemase activity. |
| تدمد: | 1756-2651 0021-924X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::7ab492844bcbc561d3b890032ffbbf2dTest https://doi.org/10.1093/oxfordjournals.jbchem.a122909Test |
| رقم الانضمام: | edsair.doi...........7ab492844bcbc561d3b890032ffbbf2d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17562651 0021924X |
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