Yeast profilin complements profilin deficiency in transgenic tomato fruits and allows development of hypoallergenic tomato fruits
| العنوان: | Yeast profilin complements profilin deficiency in transgenic tomato fruits and allows development of hypoallergenic tomato fruits |
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| المؤلفون: | Kay Foetisch, Jonas Lidholm, Yvonne Braun, Kathrin E. Paulus, Ernesto Enrique, Stefan Vieths, Sophia Sonnewald, Lien Q. Le, Daniela Weigand, Vera Mahler, Stephan Scheurer, Uwe Sonnewald |
| المصدر: | The FASEB Journal. 24:4939-4947 |
| بيانات النشر: | Wiley, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Transgene, Immunoblotting, Enzyme-Linked Immunosorbent Assay, macromolecular substances, Genetically modified crops, Biology, Biochemistry, Profilins, Solanum lycopersicum, RNA interference, Yeasts, Botany, Genetics, Humans, Genetically modified tomato, Molecular Biology, Reverse Transcriptase Polymerase Chain Reaction, Genetic Complementation Test, fungi, food and beverages, Allergens, Antigens, Plant, Immunoglobulin E, Plants, Genetically Modified, Yeast, Genetically modified organism, Complementation, Profilin, Fruit, biology.protein, Electrophoresis, Polyacrylamide Gel, RNA Interference, Biotechnology |
| الوصف: | Gene silencing of Lyc e 1 leads to reduced allergenicity of tomato fruits but impaired growth of transgenic tomato plants. The aim of the study was to restore growth of Lyc e 1-deficient tomato plants while retaining reduced allergenicity by simultaneous complementation of profilin deficiency by expression of nonallergenic yeast profilin. Transgenic plants were generated and tested by RT-PCR and immunoblotting; allergenicity of yeast profilin and transgenic fruits was investigated by IgE binding, basophil activation, and skin-prick tests. Lyc e 1 content of transgenic tomato fruits was |
| تدمد: | 1530-6860 0892-6638 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::28b5a6792b4605c1a1a214cc0dabf675Test https://doi.org/10.1096/fj.10.163063Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....28b5a6792b4605c1a1a214cc0dabf675 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15306860 08926638 |
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