Assessment of mitophagy in mt‐Keima Drosophila revealed an essential role of the PINK1‐Parkin pathway in mitophagy induction in vivo
| العنوان: | Assessment of mitophagy in mt‐Keima Drosophila revealed an essential role of the PINK1‐Parkin pathway in mitophagy induction in vivo |
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| المؤلفون: | Jongkyeong Chung, Young Yeon Kim, Hye Jin Jee, Toren Finkel, Hwan Tae Park, Da-Ye Lee, Yoon Jung Lee, Jae Sung Jang, Jeong-Hyun Yoon, Jeanho Yun, Hyunjin Kim, Young Mi Kim, Hyongjong Koh, Jee-Hyun Um, Yoon-Gu Jang |
| المصدر: | The FASEB Journal. 33:9742-9751 |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Genetically modified mouse, Kinase, Drosophila embryogenesis, PINK1, Biology, Biochemistry, Parkin, Cell biology, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, In vivo, Mitophagy, Genetics, Tensin, Molecular Biology, 030217 neurology & neurosurgery, Biotechnology |
| الوصف: | Mitophagy has been implicated in mitochondrial quality control and in various human diseases. However, the study of in vivo mitophagy remains limited. We previously explored in vivo mitophagy using a transgenic mouse expressing the mitochondria-targeted fluorescent protein Keima (mt-Keima). Here, we generated mt-Keima Drosophila to extend our efforts to study mitophagy in vivo. A series of experiments confirmed that mitophagy can be faithfully and quantitatively measured in mt-Keima Drosophila. We also showed that alterations in mitophagy upon environmental and genetic perturbation can be measured in mt-Keima Drosophila. Analysis of different tissues revealed a variation in basal mitophagy levels in Drosophila tissues. In addition, we found a significant increase in mitophagy levels during Drosophila embryogenesis. Importantly, loss-of-function genetic analysis demonstrated that the phosphatase and tensin homolog-induced putative kinase 1 (PINK1)-Parkin pathway is essential for the induction of mitophagy in vivo in response to hypoxic exposure and rotenone treatment. These studies showed that the mt-Keima Drosophila system is a useful tool for understanding the role and molecular mechanism of mitophagy in vivo. In addition, we demonstrated the essential role of the PINK1-Parkin pathway in mitophagy induction in response to mitochondrial dysfunction.-Kim, Y. Y., Um, J.-H., Yoon, J.-H., Kim, H., Lee, D.-Y., Lee, Y. J., Jee, H. J., Kim, Y. M., Jang, J. S., Jang, Y.-G., Chung, J., Park, H. T., Finkel, T., Koh, H., Yun, J. Assessment of mitophagy in mt-Keima Drosophila revealed an essential role of the PINK1-Parkin pathway in mitophagy induction in vivo. |
| تدمد: | 1530-6860 0892-6638 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::0cc0d999db35ee214983a72573bf4de6Test https://doi.org/10.1096/fj.201900073rTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........0cc0d999db35ee214983a72573bf4de6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15306860 08926638 |
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